NRPB141L

Product Name:

Phenyl NUPharose HP

Phenyl NUPharose FF and Phenyl NUPharose FF (LS) are hydrophobic chromatographic separation media formed by bonding phenyl groups on agarose gel microspheres with stable ether bonds, NUPharose are the classical agarose base microspheres biologically developed by NUPTEC. Phenyl has strong hydrophobicity, hydrophobic and π-π interaction with aromatic amino acid residues, which is suitable for the capture and moderate purification of target proteins. The ligand density of Phenyl NUPharose FF is higher than that of Phenyl NUPharose FF (LS), so it has different hydrophobicity and shows different selectivity to samples. Users can choose the appropriate product model according to the specific use scenario. Phenyl NUPharose HP is a high-resolution phenyl filler, HP is an abbreviation of High Performance. Phenyl NUPharose HP has the characteristics of high resolution and is mostly used in the pure stage. Phenyl fillers are typically used for the production of recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF), granulocyte colony-stimulating factor (G-CSF), human colony-stimulating factor (M-CSF), epidermal growth factor (EGF) and purification of monoclonal antibodies.

Product features and technical indicators

Product Name	Phenyl NUPharose HP
Catalog No.	NRPB141L, NRPB141S (Pre-packed Column)
Matrix	6% Highly cross-linked agarose
Ligand	Phenyl, ~25 μmol/mL
Particle size range^a	20~60 μm
Particle size, d50	35 μm
Dynamic binding capacity, DBC_10%^b	≥30 mg/mL
Recommended operating flow velocity	60~150 cm/h
Maximum flow velocity^c	300 cm/h
pH stability	3~13（Long-term）；2~14（CIP）
Chemical stability	Stable to common aqueous buffers, 1.0 M NaOH, 8 M Urea, 6 M guanidine hydrochloride, 30% isopropanol and 70% ethanol
Storage & Shipping	20% ethanol，2 ℃~30 ℃
Shelf life	5 years

Key words:

Phenyl NUPharose HP

Classification:

Hydrophobic interaction

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NUPTEC Inc. Ltd.

Sales Phone:0571-82872376
Business Phone:0571-82872986
E-mail:marketing@nuptec.com
Headquarters Address: Building 25, Qianwan Zhigu, Jiangnan Science and Technology City, Hangzhou City, Zhejiang Province
Production Base of Li: No.63, No.5 Road, Dangyuan, Li Town, Xiao District, Hangzhou City, Zhejiang Province
Linjiang Production Base: 698 Jing Lu, Linjiang Street, Qiantang District, Hangzhou City, Zhejiang Province

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Sales Phone：0571-82872376

Business Phone：0571-82872986

E-mail：marketing@nuptec.com