NRPB141L

Product Name:

Phenyl NUPharose HP

Phenyl NUPharose FF and Phenyl NUPharose FF (LS) are hydrophobic chromatographic separation media formed by bonding phenyl groups on agarose gel microspheres with stable ether bonds, NUPharose are the classical agarose base microspheres biologically developed by NUPTEC. Phenyl has strong hydrophobicity, hydrophobic and π-π interaction with aromatic amino acid residues, which is suitable for the capture and moderate purification of target proteins. The ligand density of Phenyl NUPharose FF is higher than that of Phenyl NUPharose FF (LS), so it has different hydrophobicity and shows different selectivity to samples. Users can choose the appropriate product model according to the specific use scenario. Phenyl NUPharose HP is a high-resolution phenyl filler, HP is an abbreviation of High Performance. Phenyl NUPharose HP has the characteristics of high resolution and is mostly used in the pure stage. Phenyl fillers are typically used for the production of recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF), granulocyte colony-stimulating factor (G-CSF), human colony-stimulating factor (M-CSF), epidermal growth factor (EGF) and purification of monoclonal antibodies.

Product features and technical indicators

Product Name	Phenyl NUPharose FF	Phenyl NUPharose FF (LS)	Phenyl NUPharose HP
Catalog No.	NRPB31L、 NRPB31S (pre-installed column)	NRPB100L、 NRPB100S (pre-mounted column)	NRPB141L、 NRPB141S (pre-mounted column)
matrix	6% cross-linked agarose		6% highly cross-linked agarose
ligand	Phenyl, ~ 45 μmol/mL	Phenyl, ~ 25 μmol/mL	Phenyl, ~ 25 μmol/mL
Particle size range^a	45 ~ 165 μm		20 ~ 60 μm
average particle size	~ 90 μm		35 μm
Dynamic binding load^b	≥35 mg/mL	≥30 mg/mL	≥30 mg/mL
recommend working flow rate	150~300 cm/h		60~150 cm/h
Maximum flow rate and pressure^c	1200 cm/h，0.3 MPa		300，0.3 MPa
pH stability	3~13 (long-term operation process),2~14 (short-term operation process such as CIP)
chemical stability	It is stable in the following solutions: commonly used aqueous buffer, 1 mol/L sodium hydroxide, 8 mol/L urea, 6 mol/L guanidine hydrochloride, 70% ethanol, etc.
Storage and transportation	20% ethanol, 2~30 ℃

Key words:

Phenyl NUPharose HP

Classification:

Hydrophobic interaction

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NUPTEC Inc. Ltd.

Sales Phone:0571-82872376
Business Phone:0571-82872986
E-mail:marketing@nuptec.com
Headquarters Address: Building 25, Qianwan Zhiqi, Jiangnan Science and Technology City, Hangzhou City, Zhejiang Province
Production Base of Li: No.63, No.5 Road, Dangyuan, Li Town, Xiao District, Hangzhou City, Zhejiang Province
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Sales Phone：0571-82872376

Business Phone：0571-82872986

E-mail：marketing@nuptec.com