NRPB129

Product Name:

Ni-NTA NUPharose Mage

Ni-NTA NUPharose Mag is a metal chelate affinity Magnetic bead resin, which is mainly used for rapid screening and purification of proteins with histidine tag (His-tag). The ligand is nitrilotriacetic acid (NTA), the coordination metal ion is Ni2 +,NUPharose Mag-based spheres are agarose gel microspheres containing paramagnetic materials. Compared with the corresponding column chromatography agarose packing (Ni-NTA NUPharose FF, catalog number NRPB57), this product has the same ligand and maintains a good balance between affinity (loading) and specificity (selectivity): the binding amount for most proteins with histidine tag (His-tag) is more than 30 mg/mL, and the purity of the sample can reach more than 90% after one-step purification, in the eluate Ni2 + drop rate is low, the molar ratio of Ni2 + and target protein is less than 0.1.

Product features and technical indicators

Product Name	Ni-NTA NUPharose Mage
Catalog No.	NRPB129
matrix	paramagnetic agarose microspheres
ligand	Nitrilotriacetic acid, ~ 15 μmol Ni2 /ml
Particle size range^a	30 to 100 μm
binding amount^b	~ 40 mg/mL Histidine-tagged protein
Use of pH	3~12 (recommend working pH),3~12 (long-term stability);2~14 (short-term stability)
chemical stability	It is stable in the following solutions: commonly used aqueous buffer; 1 mol/L sodium hydroxide; 8 mol/L urea; 6 mol/L guanidine hydrochloride; 70% ethanol.
Storage and transportation	20% Magnetic bead resin dispersion, containing 20% ethanol, stored and transported at 2~30 ℃
Shelf life	5 years

Key words:

Ni-NTA NUPharose Mage

Classification:

Magnetic bead resin

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Sales Phone：0571-82872376

Business Phone：0571-82872986

E-mail：marketing@nuptec.com