NRPB23L

Product Name:

CM NUPharose FF

Carboxymethyl (CM) is a weakly positive ion exchange agent, NUPharose as a NUPTEC biological classic agarose gel chromatography media-based sphere. Weak anion ion exchange(SP) filler has different selectivity compared with strong anion exchange filler. CM filler can be considered when SP filler cannot meet the selectivity. According to different resolutions and applicable occasions, CM NUPharose series can be divided into CM NUPharose FF(~ 90μm) and CM NUPharose HP(~ 35μm). FF is the abbreviation of Fast Flow. CM NUPharose FF has the characteristics of fast flow rate, which can be used for rapid purification of proteins, with wide applicability and easy amplification. HP is the abbreviation of High Performance. CM NUPharose HP has the characteristics of high resolution and is mostly used in the pure stage.

Product features and technical indicators

Product Name	CM NUPharose FF	CM NUPharose HP
Catalog No.	NRPB23L、 NRPB23S (pre-mounted column)	NRPB105L、 NRPB105S (pre-mounted column)
matrix	6% cross-linked agarose
functional group	Carboxymethyl,-CH2COO-
Particle size range^a	45 ~ 165 μm	20 to 50 μm
average particle size	~ 90 μm	~ 35 μm
Ion exchange容量	90~130 μmol H/mL	60~100 μmol H/mL
Dynamic binding load^b	≥90 mg/mL	≥50 mg/mL
recommend working flow rate	150~300 cm/h	60~150 cm/h
Maximum flow rate^c	1200 cm/h	300 cm/h
chemical stability	It is stable in the following solutions: common aqueous buffer, 1 M sodium hydroxide, 8 M urea, 6 M guanidine hydrochloride, 70% ethanol, etc.
pH stability	6~10 (recommend working pH);4~13 (long-term stability);3~14 (short-term stability)
Storage and transportation	20% ethanol, 2~30 ℃
Shelf life	5 years

Key words:

CM NUPharose FF

Classification:

Ion exchange

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NUPTEC Inc. Ltd.

Sales Phone:0571-82872376
Business Phone:0571-82872986
E-mail:marketing@nuptec.com
Headquarters Address: Building 25, Qianwan Zhiqi, Jiangnan Science and Technology City, Hangzhou City, Zhejiang Province
Production Base of Li: No.63, No.5 Road, Dangyuan, Li Town, Xiao District, Hangzhou City, Zhejiang Province
Linjiang Production Base: 698 Jing Lu, Linjiang Street, Qiantang District, Hangzhou City, Zhejiang Province

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Sales Phone：0571-82872376

Business Phone：0571-82872986

E-mail：marketing@nuptec.com