NRPB99L

Product Name:

Q NUPharose HP

Q NUPharose series is a strong anion Ion exchange filler, Q is a quaternary ammonium group, and the NUPharose is a NUPTEC biological classic agarose gel chromatography medium-based sphere. Compared with other Q fillers on the market, this product has the characteristics of less non-specific adsorption and less dynamic binding load affected by residence time. It is widely used in the capture, moderate purification and pure purification of biological macromolecules. According to the resolution and application of different occasions, Q NUPharose series can be divided into Q NUPharose FF(~ 90μm), Q NUPharose HP(~ 35μm), Q NUPharose BB(~ 200 μm) three. FF is an abbreviation of Fast Flow. Q NUPharose FF has the characteristics of fast flow rate, which can be used for rapid purification of proteins, with wide applicability and easy amplification. HP is the abbreviation of High Performance. Q NUPharose HP has the characteristics of high resolution and is mostly used in the pure stage. BB is the abbreviation of Big Beads. Q NUPharose BB has a large particle size and is suitable for high viscosity systems, such as blood products, and can also be used for batch static operation.

Product features and technical indicators

Product Name	Q NUPharose FF	Q NUPharose HP	Q NUPharose BB
Catalog No.	NRPB22L、 NRPB22S (pre-installed column)	NRPB99L、 NRPB99S (pre-mounted column)	NRPB101L、 NRPB101S (pre-mounted column)
matrix	6% cross-linked agarose
functional group	Quaternary ammonium,-CH2CH(OH)CH2N(CH3)3
Particle size range^a	45 ~ 165 μm	20 ~ 60 μm	100 ~ 300 μm
average particle size	~ 90 μm	~ 35 μm	~ 200 μm
Ion exchange容量	110~160 μmol OH-/mL	60~100 μmol OH-/mL	100~150 μmol OH-/mL
combining ability	≥40 mg/MLB ≥ 110 mg/MLC	≥80 mg/MLB	≥50 mg/MLD
recommend working flow rate	150~300 cm/h	60~150 cm/h	150~300 cm/h
Maximum flow rate^e	1200 cm/h	300 cm/h	1200 cm/h
chemical stability	It is stable in the following solutions: common aqueous buffer, 1 M sodium hydroxide, 8 M urea, 6 M guanidine hydrochloride, 70% ethanol, 30% isopropanol, etc.
pH stability	3~12 (recommend working pH),3~12 (long-term stability);2~14 (short-term stability)
Storage and transportation	20% ethanol, 2 ℃ ~ 30 ℃
Shelf life	5 years

Key words:

Q NUPharose HP

Classification:

Ion exchange

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NUPTEC Inc. Ltd.

Sales Phone:0571-82872376
Business Phone:0571-82872986
E-mail:marketing@nuptec.com
Headquarters Address: Building 25, Qianwan Zhiqi, Jiangnan Science and Technology City, Hangzhou City, Zhejiang Province
Production Base of Li: No.63, No.5 Road, Dangyuan, Li Town, Xiao District, Hangzhou City, Zhejiang Province
Linjiang Production Base: 698 Jing Lu, Linjiang Street, Qiantang District, Hangzhou City, Zhejiang Province

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Sales Phone：0571-82872376

Business Phone：0571-82872986

E-mail：marketing@nuptec.com