NRPB130

Product Name:

Ni-TED NUPharose Mag.

Ni-TED NUPharose Mag is a metal chelate affinity Magnetic bead resin, which is mainly used for rapid screening and purification of proteins with histidine tag (His-tag). The ligand is tricarboxymethyl ethylenediamine (tris-carboxymethyl ethylene diamine,TED) group, the coordination metal ion is Ni2 +,NUPharose Mag-based spheres are agarose gel microspheres containing paramagnetic materials. Compared with the corresponding agarose column chromatography packing (Ni-TED NUPharose FF, catalog number NRPB58), this product has the same ligand and maintains a good balance between affinity (load) and specificity (selectivity): the binding amount of protein with histidine tag (His-tag) is more than 20 mg/mL, and the purity of the sample can reach more than 90% after one-step purification, the content of Ni2 + in the eluent was consistent with the environmental background value, and the metal ion shedding rate was very low.

Product features and technical indicators

Product Name	Ni-TED NUPharose Mag.
Catalog No.	NRPB130
matrix	paramagnetic agarose microspheres
ligand	tricarboxymethylethylenediamine, chelating ~ 25 μmol Ni2 /mL
Particle size range^a	30 to 100 μm
binding amount^b	~ 20 mg/mL Histidine-tagged protein
Use of pH	3~12 (recommend working pH),3~12 (long-term stability);2~14 (short-term stability)
chemical stability	1) Purification buffer: A commonly used aqueous buffer, which can contain 10 mM EDTA,20 mM β-ME or 20 mM DTT. 2) Clean-in-place (CIP) treatment: 1 M NaOH, 1~2 M NaCl, 70% ethanol, 30% isopropanol, 0.1~0.5 non-ionic surfactant. 3) The performance of the Magnetic bead resin remained stable when treated for a specific time in the following solutions: 10 mM NaOH (long-term), 0.1 M hydrochloric acid (24 h), and 6 M guanidine hydrochloride (24 h).
Storage and transportation	20% Magnetic bead resin dispersion, containing 20% ethanol, stored and transported at 2~30 ℃
Shelf life	5 years

Key words:

Ni-TED NUPharose Mag.

Classification:

Magnetic bead resin

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Sales Phone：0571-82872376

Business Phone：0571-82872986

E-mail：marketing@nuptec.com