NRPB89L

Product Name:

Heparin NUPharose HP

Heparin NUPharose FF and Heparin NUPharose HP are heparin affinity fillers, which are bonded to heparin on the NUPharose of NUPTEC biological classic sepharose chromatography media-based spheres. FF is the abbreviation of Fast Flow, with the characteristics of fast flow rate, can be used for rapid purification of proteins, wide applicability, easy to enlarge. HP is the abbreviation of High Performance and has the characteristics of high resolution. The principle of heparin affinity is more complex, it is generally believed that the binding of heparin and protein is the result of a variety of driving forces such as ionic interactions, hydrogen bonds and van der Waals forces. Heparin filler is mainly used for the separation and purification of thrombin III, coagulation factor, lipoprotein, esterase, hormone, interferon, etc. The use of heparin filler for exosome purification has also been reported in the literature. NUPTEC biological provides a series of recombinant growth factor products, the use of heparin filler purification of various production factors experience, can provide users with excellent performance of heparin filler products and services. These two products retain the excellent hydrophilicity and large mesh structure of agarose, and have good compatibility with biologically active macromolecules, with high load, less non-specific adsorption, fast flow rate and other characteristics, suitable for a variety of heparin Affinity chromatograph.

Product features and technical indicators

Product Name	Heparin NUPharose HP
Catalog No.	NRPB89L, NRPB89S (pre-installed column)
matrix	6% highly cross-linked agarose
ligand	Porcine Heparin
Particle size range^a	20 ~ 60 μm
average particle size	~ 35 μm
combining ability^b	≥8 mg afgf/ml
recommend working flow rate	60~150 cm/h
Maximum flow rate and pressure^c	300 cm/h，0.3 MPa
Use of pH	4~12 (long-term stability),4~13 (short-term operation such as CIP)
chemical stability	It is stable in the common aqueous buffer, 0.5 mol/L sodium hydroxide, 8 mol/L urea, 30% isopropanol and 70% ethanol.
Storage and transportation	20% ethanol (including 0.05 M NaAc),2~30 ℃
Shelf life	3 years

Key words:

Heparin NUPharose HP

Classification:

Affinity purification

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NUPTEC Inc. Ltd.

Sales Phone:0571-82872376
Business Phone:0571-82872986
E-mail:marketing@nuptec.com
Headquarters Address: Building 25, Qianwan Zhigu, Jiangnan Science and Technology City, Hangzhou City, Zhejiang Province
Guali Production Base: No. 63, Yuanwu Road, Dangshan Industrial Park, Guali Town, Xiaoshan District, Hangzhou City, Zhejiang Province
Linjiang Production Base: 698 Jing Lu, Linjiang Street, Qiantang District, Hangzhou City, Zhejiang Province

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Sales Phone：0571-82872376

Business Phone：0571-82872986

E-mail：marketing@nuptec.com