NUPTEC Inc. Ltd.

Product Details

Professional Manufaturer of Resins for Protein Purification

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Heparin NUPharose HP
Heparin NUPharose HP
Heparin NUPharose HP
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  • Heparin NUPharose HP
  • Heparin NUPharose HP
  • Heparin NUPharose HP

Catalog No:

NRPB89L

Product Name:

Heparin NUPharose HP


Heparin NUPharose FF and Heparin NUPharose HP are heparin affinity fillers, which are bonded to heparin on the NUPharose of NUPTEC biological classic sepharose chromatography media-based spheres. FF is the abbreviation of Fast Flow, with the characteristics of fast flow rate, can be used for rapid purification of proteins, wide applicability, easy to enlarge. HP is the abbreviation of High Performance and has the characteristics of high resolution. The principle of heparin affinity is more complex, it is generally believed that the binding of heparin and protein is the result of a variety of driving forces such as ionic interactions, hydrogen bonds and van der Waals forces. Heparin filler is mainly used for the separation and purification of thrombin III, coagulation factor, lipoprotein, esterase, hormone, interferon, etc. The use of heparin filler for exosome purification has also been reported in the literature. NUPTEC biological provides a series of recombinant growth factor products, the use of heparin filler purification of various production factors experience, can provide users with excellent performance of heparin filler products and services. These two products retain the excellent hydrophilicity and large mesh structure of agarose, and have good compatibility with biologically active macromolecules, with high load, less non-specific adsorption, fast flow rate and other characteristics, suitable for a variety of heparin Affinity chromatograph.

Product features and technical indicators

Product Name

Heparin NUPharose HP

Catalog No.

NRPB89L, NRPB89S (pre-installed column)

matrix

6% highly cross-linked agarose

ligand

Porcine Heparin

Particle size rangea

20 ~ 60 μm

average particle size

~ 35 μm

combining abilityb

≥8 mg afgf/ml

recommend working flow rate

60~150 cm/h

Maximum flow rate and pressurec

300 cm/h,0.3 MPa

Use of pH

4~12 (long-term stability),4~13 (short-term operation such as CIP)

chemical stability

It is stable in the common aqueous buffer, 0.5 mol/L sodium hydroxide, 8 mol/L urea, 30% isopropanol and 70% ethanol.

Storage and transportation

20% ethanol (including 0.05 M NaAc),2~30 ℃

Shelf life

3 years