NRPB74L

Product Name:

IDA NUPharose FF

Ni-IDA NUPharose FF and IDA NUPharose FF is a metal chelating filler containing iminodiacetic acid (iminodiacetic acid,IDA) group, which is suitable for most Affinity chromatograph with histidine tag (His-tag) protein. Both products are based on NUPTEC biological classic NUPharose agarose gel microspheres. FF is an abbreviation of Fast Flow, which means high flow rate. IDA NUPharose FF does not chelate metal ions, users can chelate Ni2 +, Co2 +, Cu2 +, Zn2 + and other metal ions after use; Ni-IDA NUPharose FF has chelated Ni2 +, users can directly use. Through the unique ligand modification process, the two products have achieved a balance of affinity and specificity: the binding amount of most histidine-tagged proteins is more than 40 mg/mL, and the purity of the sample can reach more than 90% after one-step purification.

Product features and technical indicators

Product Name	IDA NUPharose FF
Catalog No.	NRPB74L, NRPB74S (pre-installed column)
matrix	6% cross-linked agarose
ligand	Iminodiacetic acid, chelating ~ 30 μmol Ni²/mL
Particle size range^a	45 ~ 165 μm
average particle size	~ 90 μm
Dynamic binding load^b	Histidine-tagged protein ≥ 40 mg/mL
recommend working flow rate^c	60~300 cm/h
Maximum flow rate and pressure^d	1500 cm/h，0.5 MPa
Use of pH	4~8.5 (recommend working pH),3~12 (long-term stability);2~14 (short-term stability)
chemical stability	It is stable in the following solutions: commonly used aqueous buffer, 1 mol/L sodium hydroxide, 8 mol/L urea, 6 mol/L guanidine hydrochloride, 70% ethanol, etc.
Storage and transportation	20% ethanol, 2~30 ℃
Shelf life	5 years

Key words:

IDA NUPharose FF

Classification:

Affinity purification

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NUPTEC Inc. Ltd.

Sales Phone:0571-82872376
Business Phone:0571-82872986
E-mail:marketing@nuptec.com
Headquarters Address: Building 25, Qianwan Zhiqi, Jiangnan Science and Technology City, Hangzhou City, Zhejiang Province
Production Base of Li: No.63, No.5 Road, Dangyuan, Li Town, Xiao District, Hangzhou City, Zhejiang Province
Linjiang Production Base: 698 Jing Lu, Linjiang Street, Qiantang District, Hangzhou City, Zhejiang Province

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Sales Phone：0571-82872376

Business Phone：0571-82872986

E-mail：marketing@nuptec.com