NUPTEC Inc. Ltd.

Product Details

Professional Manufaturer of Resins for Protein Purification

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Butyl-S NUPharose FF
Butyl-S NUPharose FF
Butyl-S NUPharose FF
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  • Butyl-S NUPharose FF
  • Butyl-S NUPharose FF
  • Butyl-S NUPharose FF

Catalog No:

NRPB96L

Product Name:

Butyl-S NUPharose FF


Butyl-S (butyl sulfide) filler is a hydrophobic chromatography medium formed by modifying a spacer arm with a length of twelve atoms on the surface of the agarose gel microsphere skeleton, and then coupling the n-butyl group through a thioether bond. The ligand design of butyl sulfide is to show different selectivity with other hydrophobic chromatography media. The main principle is that different target molecules have different interactions with sulfur atoms, and the corresponding chromatography categories can be subdivided into thiophilic hydrophobic chromatography. Butyl-S fillers are widely used in the separation, purification and renaturation of proteins, as well as in the purification of enzymes, virus particles, antibodies and nucleic acids. NUPTEC Bio offers two Butyl-S fillers Butyl-S NUPharose FF and NuPerley Butyl-S. Butyl-S NUPharose FF is based on NuPharose technology platform, which has the characteristics of good compatibility between agarose matrix and bioactive macromolecules, less non-specific adsorption and fast flow rate. NuPerley Butyl-S is based on a new generation of high rigidity agarose NuPerley technology platform, which has the characteristics of high rigidity, high flow rate and high load on the basis of NUPharose technology.

Product features and technical indicators

Product Name

Butyl-S NUPharose FF

NuPerley Butyl-S

Catalog No.

NRPB96L, NRPB96S (pre-installed column)

NRPB95L, NRPB95S (pre-installed column)

matrix

6% cross-linked agarose

high rigidity agarose

ligand

Butyl sulfide, a spacer arm of twelve atoms in length

ligand density

~10 μmol/mL

~15 μmol/mL

Particle size rangea

45 ~ 165 μm

30 to 100 μm

average particle size

~ 90 μm

~ 60 μm

recommend working flow rate

150~300 cm/h

Maximum flow rate and pressureb

900 cm/h,0.3 MPa

1500 cm/h,0.5 MPa

pH stability

3~13 (long-term operation process),2~14 (short-term operation process such as CIP)

chemical stability

It is stable in the following solutions: commonly used aqueous buffer, 1 mol/L sodium hydroxide, 8 mol/L urea, 6 mol/L guanidine hydrochloride, 70% ethanol, etc.

Storage and transportation

20% ethanol, 2~30 ℃