NRPB13L

Product Name:

PAG NUPharose FF affinity filler

The PAG NUPharose FF is an affinity separation medium formed by binding a recombinant Protein A Protein G fusion protein (r-PAG, Nuptec NRPA14) onto a sepharose microsphere. Compared with a separate protein A or protein G separation medium, it has a broader binding range, can bind to all human IgG subtypes, IgA, IgE, and IgM, but cannot bind to mouse IgA, IgM and serum albumin, and is suitable for the extraction and detection of mouse IgG monoclonal antibody. At the same time the fused r-PAG reduces the dependence on pH, allowing binding at pH5-8.

Product features and technical indicators

Product Name	PAG NUPharose FF
Catalog No.	NRPB13L, NRPB13S (pre-installed column)
matrix	4% cross-linked agarose
ligand	Protein A Protein G fusion protein, ≥ 6 mg/mL
Particle size range^a	45 ~ 165 μm
average particle size	~ 90 μm
combining ability^b	≥40 mg H-igg/ml
recommend working flow rate	60~150 cm/h
Maximum flow rate and pressure^c	900 cm/h，0.3 MPa
Use of pH	pH 3~9 (long term),pH 2~10 (short term)
Storage and transportation	20% ethanol, 2~8 ℃ storage, 2~30 ℃ transportation
Shelf life	3 years

Key words:

PAG NUPharose FF affinity filler

Classification:

Affinity purification

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E-mail: marketing@nuptec.com

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NUPTEC Inc. Ltd.

Sales Phone:0571-82872376
Business Phone:0571-82872986
E-mail:marketing@nuptec.com
Headquarters Address: Building 25, Qianwan Zhiqi, Jiangnan Science and Technology City, Hangzhou City, Zhejiang Province
Production Base of Li: No.63, No.5 Road, Dangyuan, Li Town, Xiao District, Hangzhou City, Zhejiang Province
Linjiang Production Base: 698 Jing Lu, Linjiang Street, Qiantang District, Hangzhou City, Zhejiang Province

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Sales Phone：0571-82872376

Business Phone：0571-82872986

E-mail：marketing@nuptec.com