Product Details
Professional Manufaturer of Resins for Protein Purification
Catalog No:
NRPB23L
Product Name:
CM NUPharose FF
Carboxymethyl (CM) is a weakly positive ion exchange agent, NUPharose as a NUPTEC biological classic agarose gel chromatography media-based sphere. Weak anion ion exchange(SP) filler has different selectivity compared with strong anion exchange filler. CM filler can be considered when SP filler cannot meet the selectivity.
According to different resolutions and applicable occasions, CM NUPharose series can be divided into CM NUPharose FF(~ 90μm) and CM NUPharose HP(~ 35μm). FF is the abbreviation of Fast Flow. CM NUPharose FF has the characteristics of fast flow rate, which can be used for rapid purification of proteins, with wide applicability and easy amplification. HP is the abbreviation of High Performance. CM NUPharose HP has the characteristics of high resolution and is mostly used in the pure stage.
Product features and technical indicators
Product Name | CM NUPharose FF |
Catalog No. | NRPB23L, NRPB23S (Pre-packed Column) |
Matrix | 6% Cross-linked agarose |
Ligand | Carboxymethyl, -CH2COO- |
Particle size rangea | 45~165 μm |
Particle size, d50 | ~90 μm |
Ionic capacity | 90~130 μmol H+ /mL |
Dynamic binding capacity, DBC10%b | ≥90 mg/mL |
Recommended operating flow velocity | 150~300 cm/h |
Maximum flow velocityc | 1200 cm/h |
pH stability | 6~10(Recommended operating, pH),4~13(Long-term);3~14(CIP) |
Chemical stability | Stable to common aqueous buffers, 1.0 M NaOH, 8 M Urea, 6 M guanidine hydrochloride, 30% isopropanol and 70% ethanol |
Storage & Shipping | 20% ethanol, 2℃~30 ℃ |
Shelf life | 5 years |
Key words:
CM NUPharose FF
Classification:
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