NUPTEC Inc. Ltd.

Product Details

Professional Manufaturer of Resins for Protein Purification

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CM NUPharose FF
CM NUPharose FF
CM NUPharose FF
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  • CM NUPharose FF
  • CM NUPharose FF
  • CM NUPharose FF

Catalog No:

NRPB23L

Product Name:

CM NUPharose FF


Carboxymethyl (CM) is a weakly positive ion exchange agent, NUPharose as a NUPTEC biological classic agarose gel chromatography media-based sphere. Weak anion ion exchange(SP) filler has different selectivity compared with strong anion exchange filler. CM filler can be considered when SP filler cannot meet the selectivity. According to different resolutions and applicable occasions, CM NUPharose series can be divided into CM NUPharose FF(~ 90μm) and CM NUPharose HP(~ 35μm). FF is the abbreviation of Fast Flow. CM NUPharose FF has the characteristics of fast flow rate, which can be used for rapid purification of proteins, with wide applicability and easy amplification. HP is the abbreviation of High Performance. CM NUPharose HP has the characteristics of high resolution and is mostly used in the pure stage.

Product features and technical indicators

Product Name

CM NUPharose FF

Catalog No.

NRPB23L, NRPB23S (Pre-packed Column)
Matrix
6% Cross-linked agarose
Ligand
Carboxymethyl, -CH2COO-
Particle size rangea
45~165 μm
Particle size, d50
~90 μm
Ionic capacity
90~130 μmol H+ /mL
Dynamic binding capacity, DBC10%b
≥90 mg/mL
Recommended operating flow velocity
150~300 cm/h
Maximum flow velocityc
1200 cm/h
pH stability
6~10(Recommended operating, pH),4~13(Long-term);3~14(CIP)
Chemical stability
Stable to common aqueous buffers, 1.0 M NaOH, 8 M Urea, 6 M guanidine hydrochloride, 30% isopropanol and 70% ethanol
Storage & Shipping
20% ethanol, 2℃~30 ℃

Shelf life

5 years