NRPB74L

Product Name:

IDA NUPharose FF

Ni-IDA NUPharose FF and IDA NUPharose FF is a metal chelating filler containing iminodiacetic acid (iminodiacetic acid,IDA) group, which is suitable for most Affinity chromatograph with histidine tag (His-tag) protein. Both products are based on NUPTEC biological classic NUPharose agarose gel microspheres. FF is an abbreviation of Fast Flow, which means high flow rate. IDA NUPharose FF does not chelate metal ions, users can chelate Ni2 +, Co2 +, Cu2 +, Zn2 + and other metal ions after use; Ni-IDA NUPharose FF has chelated Ni2 +, users can directly use. Through the unique ligand modification process, the two products have achieved a balance of affinity and specificity: the binding amount of most histidine-tagged proteins is more than 40 mg/mL, and the purity of the sample can reach more than 90% after one-step purification.

Product features and technical indicators

Product Name	IDA NUPharose FF
Catalog No.	NRPB74L, NRPB74S (pre-packed column)
Matrix	Cross-linked agarose, 6%
Ligand	Iminodiacetic acid, precharged with ~30 μmol Ni²⁺/mL
Particle size range^a	45 ~ 165 μm
Particle size, d₅₀	~ 90 μm
Dynamic binding capacity, DBC_10%^b	≥40 mg his-tagged protein/mL
Recommended operating flow velocity^c	60~300 cm/h
Maximum flow velocityd	1500 cm/h，0.5 MPa
pH stability	4~8.5 (recommend working pH),3~12 (long-term);2~14 (short-term)
Chemical stability	Stable to common aqueous buffers: 1 M NaOH, 8 M urea, 6 M guanidine hydrochloride, 70% ethanol.
Storage & Shipping	20% ethanol, 2~30 ℃
Shelf life	5 years

Key words:

IDA NUPharose FF

Classification:

Affinity purification

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NUPTEC Inc. Ltd.

Sales Phone:0571-82872376
Business Phone:0571-82872986
E-mail:marketing@nuptec.com
Headquarters Address: Building 25, Qianwan Zhigu, Jiangnan Science and Technology City, Hangzhou City, Zhejiang Province
Guali Production Base: No. 63, Yuanwu Road, Dangshan Industrial Park, Guali Town, Xiaoshan District, Hangzhou City, Zhejiang Province
Linjiang Production Base: 698 Jing Lu, Linjiang Street, Qiantang District, Hangzhou City, Zhejiang Province

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Sales Phone：0571-82872376

Business Phone：0571-82872986

E-mail：marketing@nuptec.com