NRPB94L

Product Name:

NuPerley Q HR

NuPerley Q HR is a strong anion exchange chromatography medium with high resolution and high flow rate. It is composed of quaternary ammonium groups (Q) and a new generation of small particle size, high rigidity agarose gel microspheres NuPerley bonded. The average particle size of this product is 35 μm, and the flow rate of more than 900 cm/h can still be achieved. Thanks to the optimization of Ion exchange capacity (ligand density), the dynamic binding load of NuPerley Q HR to BSA is more than 60 mg/mL at a residence time of 2 min, which is higher than that of traditional Q NUPharose FF, and is mainly used for moderate purification and purity. When the purification scale is not very large, or the initial sample is relatively clean (such as supernatant expression and high expression of samples), this product can also be used to achieve high purity through one-step purification.

Product features and technical indicators

Product Name	NuPerley Q HR
Catalog No.	NRPB94L, NRPB94S (pre-installed column)
matrix	High resolution, high rigidity agarose microspheres
functional group	-CH2CH(OH)CH2N(CH3)3
average particle size	~ 35 μm
Ion exchange容量	40~80 μmol OH-/mL
Dynamic binding load^a	≥60 mg/mL
recommend working flow rate	50~150 cm/h
Maximum flow rate^b	900 cm/h
chemical stability	It is stable in the following solutions: common aqueous buffer, 1 M sodium hydroxide, 8 M urea, 6 M guanidine hydrochloride, 70% ethanol, 30% isopropanol, etc.
pH stability	3~12 (recommend working pH),3~12 (long-term stability);2~14 (short-term stability)
Storage and transportation	20% ethanol, 2 ℃ ~ 30 ℃
Shelf life	5 years

Key words:

NuPerley Q HR

Classification:

Ion exchange

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E-mail: marketing@nuptec.com

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NUPTEC Inc. Ltd.

Sales Phone:0571-82872376
Business Phone:0571-82872986
E-mail:marketing@nuptec.com
Headquarters Address: Building 25, Qianwan Zhigu, Jiangnan Science and Technology City, Hangzhou City, Zhejiang Province
Guali Production Base: No. 63, Yuanwu Road, Dangshan Industrial Park, Guali Town, Xiaoshan District, Hangzhou City, Zhejiang Province
Linjiang Production Base: 698 Jing Lu, Linjiang Street, Qiantang District, Hangzhou City, Zhejiang Province

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Sales Phone：0571-82872376

Business Phone：0571-82872986

E-mail：marketing@nuptec.com