NRPB116L

Product Name:

NuPearla Sulfate

NuPearla Sulfate is a Affinity chromatograph separation medium formed after the direct sulfation of cellulose microspheres. NuPearla is a cellulose matrix microsphere introduced by NUPTEC biology, which belongs to the "soft glue" of natural polymer source like agarose matrix microsphere ". Compared with agarose, the raw materials of cellulose microspheres have standard grades, the cellulose skeleton does not contain anionic groups, and the non-specific adsorption of cellulose microspheres is 1/10 to 1/3 of that of agarose microspheres. For example, the non-specific adsorption of cytochrome C by cellulose-based microspheres of this product can be as low as 2 μg/mL. After sulfation, NuPearla Sulfate have affinity for a variety of live viruses, inactivated or structurally cleaved viruses, viral or microbial antigens and heparin binding proteins. At the same time, sulfate is an excellent salt-tolerant cation ion exchange ligand, NuPearla Sulfate can also be used as a cation ion exchange filler, the binding amount of lysozyme can reach 180 mg/mL, and in 50~200 mM NaCl salt concentration is still good binding.

Product features and technical indicators

Product Name	NuPearla Sulfate
Catalog No.	NRPB116L, NRPB116S (pre-installed column)
matrix	highly cross-linked cellulose
ligand	Sulfate, 160~200 μmol H /mL
Particle size range^a	45 ~ 165 μm
average particle size	~ 90 μm
combining ability^b	120 mg/mL
recommend working flow rate	60~150 cm/h
Maximum flow rate and pressure^c	300 cm/h，0.3 MPa
Use of pH	4~8.5 (recommend working pH),3~12 (long-term stability);2~14 (short-term stability)
chemical stability	Stable in the following solutions: commonly used aqueous buffer, 0.5 mol/L sodium hydroxide, etc.
Storage and transportation	20% ethanol, 2~30 ℃
Shelf life	5 years

Key words:

NuPearla Sulfate

Classification:

Affinity purification

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E-mail: marketing@nuptec.com

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NUPTEC Inc. Ltd.

Sales Phone:0571-82872376
Business Phone:0571-82872986
E-mail:marketing@nuptec.com
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Sales Phone：0571-82872376

Business Phone：0571-82872986

E-mail：marketing@nuptec.com