NRPB114L

Product Name:

NuPerley DexS-HbP

NuPerley DexS-HbP and NuPerley DexS-VirS is a Affinity chromatograph separation medium formed by bonding dextran sulfate (Dextran Sulfate) to a new generation of highly rigid agarose microsphere NuPerley. Dextran sulfate, also known as dextran sulfate, is a synthetic derivative of natural polysaccharide dextran, which has similar biological activity with heparin. It is a non-animal source heparin-like ligand, and the filler product is called heparin-like affinity filler. NuPerley DexS-HbP has a relatively low sulfur content (40-80 μmol/mL) and can be used for affinity separation and purification of proteins, such as plasma proteins. At the same time, sulfate is an excellent salt-tolerant cation ion exchange ligand, NuPerley DexS-HbP can also be used as a cation ion exchange filler, the binding amount of lysozyme is more than 60 mg/mL, and in 50~150 mM NaCl salt concentration is still good binding. NuPerley DexS-VirS has a relatively high sulfur content (70-130 μmol/mL) and is mainly used for the capture and purification of viruses and virus-like particles (VLPs).

Product features and technical indicators

Product Name	NuPerley DexS-HbP	NuPerley DexS-VirS
Catalog No.	NRPB114L NRPB114S (pre-mounted column)	NRPB115L NRPB115S (pre-mounted column)
matrix	high rigidity agarose
ligand	Dextran sulfate, sulfur content 40~80 μmol/mL	Dextran sulfate, sulfur content 70~130 μmol/mL
Particle size range^a	30 to 100 μm
average particle size	~ 60 μm
combining ability^b	≥ 60 mg lysozyme/mL	~ 100 mg lysozyme/mL
recommend working flow rate	60~300 cm/h
Maximum flow rate and pressure^c	1500 cm/h，0.3 MPa
Use of pH	4~13 (long-term stability),3~14 (short-term operation such as CIP)
chemical stability	It is stable in the common aqueous buffer, 0.5 mol/L sodium hydroxide, 8 mol/L urea, 30% isopropanol and 70% ethanol.
Storage and transportation	20% ethanol, 2~8 ℃
Shelf life	3 years

Key words:

NuPerley DexS-HbP

Classification:

Affinity purification

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Sales Phone：0571-82872376

Business Phone：0571-82872986

E-mail：marketing@nuptec.com