NRPB134L

Product Name:

NuPerley Ni-NTA

NuPerley IMAC and NuPerley Ni-NTA are metal chelating fillers containing nitrilotriacetic acid (NTA) groups, which are mainly used for Affinity chromatograph of histidine-tagged (His-tag) proteins, and are NuPerley as a new generation of high rigidity agarose gel base microspheres developed for NUPTEC biology. NuPerley IMAC does not chelate metal ions, users can chelate Ni2, Co2, Cu2, Zn2 and other metal ions after use, NuPerley Ni-NTA has chelated Ni2, users can directly use.

Product features and technical indicators

Product Name	NuPerley IMAC	NuPerley Ni-NTA
Catalog No.	NRPB112L, NRPB112S (pre-installed column)	NRPB134L, NRPB134S (pre-installed column)
matrix	high rigidity agarose
ligand	Nitrilotriacetic acid, chelating ~ 23 μmol Ni2 /ml	Nitrilotriacetic acid, pre-chelated ~ 23 μmol Ni2 /ml
Particle size range^a	30 to 100 μm
average particle size	~ 60 μm
Dynamic binding load^b	~ 50 mg/mL Histidine-tagged protein
recommend working flow rate	60~300 cm/h
Maximum flow rate and pressure^c	1500 cm/h，0.5 MPa
pH stability	3~12 (recommend working pH),3~12 (long-term stability);2~14 (short-term stability) d
chemical stability	It is stable in the following solutions: commonly used aqueous buffer, 1 mol/L sodium hydroxide, 8 mol/L urea, 6 mol/L guanidine hydrochloride, 70% ethanol, etc. The effects of common reducing agents, chelating agents, detergents, denaturants, etc. on fillers are shown in the table below.
Storage and transportation	20% ethanol, 2~30 ℃
Shelf life	5 years

Key words:

NuPerley Ni-NTA

Classification:

Affinity purification

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NUPTEC Inc. Ltd.

Sales Phone:0571-82872376
Business Phone:0571-82872986
E-mail:marketing@nuptec.com
Headquarters Address: Building 25, Qianwan Zhigu, Jiangnan Science and Technology City, Hangzhou City, Zhejiang Province
Guali Production Base: No. 63, Yuanwu Road, Dangshan Industrial Park, Guali Town, Xiaoshan District, Hangzhou City, Zhejiang Province
Linjiang Production Base: 698 Jing Lu, Linjiang Street, Qiantang District, Hangzhou City, Zhejiang Province

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Sales Phone：0571-82872376

Business Phone：0571-82872986

E-mail：marketing@nuptec.com