NRPB122L

Product Name:

Ni-NTA NUPharose HP

NuPerley IDA is a metal chelating filler containing iminodiacetic acid (iminodiacetic acid,IDA) group, which is suitable for most Affinity chromatograph with histidine tag (His-tag) protein, and is NuPerley as a new generation of high rigidity agarose gel base microspheres developed for NUPTEC biology. NuPerley IDA does not chelate metal ions, users can chelate Ni2 +, Co2 +, Cu2 +, Zn2 + and other metal ions after use. Compared with the IDA NUPharose FF with an average particle size of 90 μm, this product has an average particle size of ~ 60 μm, which has a significantly improved resolution and can withstand higher column pressures. Through a unique ligand modification process, NuPerley IDA products have achieved a balance of affinity and specificity: the binding amount of most histidine-tagged proteins is more than 40 mg/mL, and the purity of the sample can reach more than 90% after one-step purification. This product takes into account the high load, high rigidity, high flow rate, high specificity and higher resolution, providing more abundant affinity and specificity selection.

Product features and technical indicators

Product Name	IMAC NUPharose HP	Ni-NTA NUPharose HP
Catalog No.	NRPB111L, NRPB111S (pre-installed column)	NRPB122L, NRPB122S (pre-installed column)
matrix	6% highly cross-linked agarose
ligand	nitrilotriacetic acid, Chelatable ~ 17 μmol Ni2 /ml	nitrilotriacetic acid, Chelated ~ 17 μmol Ni2 /ml
Particle size range^a	20 to 50 μm
average particle size	~ 35 μm
Dynamic binding load^b	Histidine-tagged protein ≥ 50 mg/mL
recommend working flow rate	60~150 cm/h
Maximum flow rate and pressure^c	300 cm/h，0.5 MPa
pH stability	3~12 (recommend working pH),3~12 (long-term stability);2~14 (short-term stability) d
chemical stability	It is stable in the following solutions: commonly used aqueous buffer, 1 mol/L sodium hydroxide, 8 mol/L urea, 6 mol/L guanidine hydrochloride, 70% ethanol, etc. The effects of common reducing agents, chelating agents, detergents, denaturants, etc. on fillers are shown in the table below.
Storage and transportation	20% ethanol, 2~30 ℃
Shelf life	5 years

Key words:

Ni-NTA NUPharose HP

Classification:

Affinity purification

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NUPTEC Inc. Ltd.

Sales Phone:0571-82872376
Business Phone:0571-82872986
E-mail:marketing@nuptec.com
Headquarters Address: Building 25, Qianwan Zhigu, Jiangnan Science and Technology City, Hangzhou City, Zhejiang Province
Guali Production Base: No. 63, Yuanwu Road, Dangshan Industrial Park, Guali Town, Xiaoshan District, Hangzhou City, Zhejiang Province
Linjiang Production Base: 698 Jing Lu, Linjiang Street, Qiantang District, Hangzhou City, Zhejiang Province

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Sales Phone：0571-82872376

Business Phone：0571-82872986

E-mail：marketing@nuptec.com