NRPB51L

Product Name:

Co-NTA NUPharose FF

Co-NTA NUPharose FF is a metal chelating filler containing nitrilotriacetic acid (NTA) group, which is mainly used for Affinity chromatograph of histidine tag (His-tag) protein. This product is based on NUPTEC biological classic NUPharose agarose gel microspheres, pre-chelated Co2 on the basis of IMAC NUPharose FF (catalog number NRPB76), and can be used directly by users. The affinity and specificity of different metal ions to the target protein are different. The affinity of Cu2, Ni2, Zn2 and Co2 decreases in turn (affecting the yield), and the specificity increases in turn (affecting the purity). When the chelated Ni2 cannot meet the purity requirements, the chelated metal ion Co2 of this product can be considered. Compared with iminodiacetic acid (IDA) ligand, NTA ligand has more balanced performance, better tolerance to chelating agents, reducing agents, alkali and other reagents, and less metal ions are shed from the eluent during purification.

Product features and technical indicators

Product Name	Co-NTA NUPharose FF
Catalog No.	NRPB51L, NRPB51S (pre-installed column)
matrix	6% cross-linked agarose
ligand	Nitrilotriacetic acid, ~ 17 μmol Co²/ml
Particle size range^a	45 ~ 165 μm
average particle size	~ 90 μm
Dynamic binding load^b	Histidine-tagged protein ≥ 40 mg/mL
recommend working flow rate	60~300 cm/h
Maximum flow rate and pressure^c	1500 cm/h，0.5 MPa
PH stability	3~12 (recommend working pH),3~12 (long-term stability);2~14 (short-term stability)^d
chemical stability	It is stable in the following solutions: commonly used aqueous buffer, 1 mM EDTA, 1 mol/L sodium hydroxide, 8 mol/L urea, 6 mol/L guanidine hydrochloride, 70% ethanol, etc.
Storage and transportation	20% ethanol, 2~30 ℃
Shelf life	5 years

Key words:

Co-NTA NUPharose FF

Classification:

Affinity purification

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NUPTEC Inc. Ltd.

Sales Phone:0571-82872376
Business Phone:0571-82872986
E-mail:marketing@nuptec.com
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Sales Phone：0571-82872376

Business Phone：0571-82872986

E-mail：marketing@nuptec.com