NRPB17L

Product Name:

Dextrin NUPharose FF

The Dextrin NUPharose FF is a Affinity chromatograph separation medium formed by bonding dextrins to NUPharose agarose-based balls. The dextrin ligand can specifically bind to maltose binding protein (MBP), so that the fusion protein with MBP tag can be purified. This product can purify the target protein under close to physiological conditions, and can retain the activity of the target protein to the greatest extent. The MBP tag is large and has a molecular weight of about 42 kDa. The main function of MBP tag is to promote the correct folding of the target protein, increase the expression level of the protein, and improve the solubility of the target protein. At the same time, the fusion protein with MBP tag specific affinity filler, MBP tag is often used in E. coli system, and the tag is usually removed by specific enzyme after Affinity chromatograph.

Product features and technical indicators

Product Name	Dextrin NUPharose FF
Catalog No.	NRPB17L, NRPB17S (pre-installed column)
matrix	6% cross-linked agarose
ligand	dextrin
Particle size range^a	45 ~ 165 μm
average particle size	~ 90 μm
comprehensive ability^b	3-5 mg maltose binding protein (about 42kDa)/mL
recommend working flow rate	60~150 cm/h
Maximum flow rate and pressure^c	900cm/h,0.3Mpa
Use of PH	7~9 (recommend working pH),2~13(CIP cleaning)
chemical stability	Stable in the following solutions: common aqueous buffer, 0.5 mol/L NaOH.
Storage and transportation	20% ethanol, 2~8 ℃ (storage),2~30 ℃ (transportation)
Shelf life	3 years

Key words:

Dextrin NUPharose FF

Classification:

Affinity purification

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E-mail: marketing@nuptec.com

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NUPTEC Inc. Ltd.

Sales Phone:0571-82872376
Business Phone:0571-82872986
E-mail:marketing@nuptec.com
Headquarters Address: Building 25, Qianwan Zhigu, Jiangnan Science and Technology City, Hangzhou City, Zhejiang Province
Production Base of Li: No.63, No.5 Road, Dangyuan, Li Town, Xiao District, Hangzhou City, Zhejiang Province
Linjiang Production Base: 698 Jing Lu, Linjiang Street, Qiantang District, Hangzhou City, Zhejiang Province

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Sales Phone：0571-82872376

Business Phone：0571-82872986

E-mail：marketing@nuptec.com