NUPTEC Inc. Ltd.

Product Details

Professional Manufaturer of Resins for Protein Purification

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NHS Activated NUPharose FF
NHS Activated NUPharose FF
NHS Activated NUPharose FF
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  • NHS Activated NUPharose FF
  • NHS Activated NUPharose FF
  • NHS Activated NUPharose FF

Catalog No:

NRPB11L

Product Name:

NHS Activated NUPharose FF


NHS Activated NUPharose FF pre-activated filler is a pre-activated separation medium formed by activating carboxyl groups with N-hydroxysuccinimide (NHS) after modifying spacer arms with a length of ten atoms on the surface of agarose gel microsphere skeleton. NuPerley NHS HR is a pre-activated filler based on a new generation of high-rigidity agarose NuPerley technology platform with higher resolution. NHS activation groups can be coupled with functional biological macromolecules containing primary amines such as antibodies, proteins, enzymes, polypeptides, etc. to form stable amide bonds, and the obtained Affinity chromatograph separation medium can specifically bind to the target molecules, and high purity products can be obtained by one-step purification. Therefore, NHS pre-activation fillers are widely used in scientific research and production fields.

Product features and technical indicators

Product Name

NHS Activated NUPharose FF

Catalog No.

NRPB11L,NRPB11S (pre-mounted column)

matrix

4% highly cross-linked agarose

Particle size rangea

45 ~ 165 μm

average particle size

~ 90 μm

activated ligand

NHS, ≥10 μmol/mL

recommend working flow rate

60~300 cm/h

Maximum flow rate and pressureb

900 cm/h,0.3 MPa

Use of pH

6~9 (coupling buffer)

Storage and transportation

100% isopropanol,-25 to -10°C (storage),2 to 8°C (transport)

Shelf life 1 year