NRPB76L

Product Name:

IMAC NUPharose FF

Ni-NTA NUPharose FF and IMAC NUPharose FF are metal chelating fillers containing nitrilotriacetic acid (NTA) groups, which are mainly used for Affinity chromatograph of histidine-tagged (His-tag) proteins. These two products are based on NUPTEC biological classic NUPharose agarose gel microspheres: IMAC NUPharose FF unchelated metal ions, and users can chelate Ni2 +, Co2 +, Cu2 +, Zn2 + and other metal ions after use; Ni-NTA NUPharose FF has chelated Ni2 +, users can directly use it. Through the optimization of the ligand modification process, the two products have achieved a balance of affinity and specificity: the binding amount of histidine-tagged protein is more than 40 mg/mL, and the purity of the sample can reach more than 90% after one-step purification. In addition to histidine, metal chelating fillers can also form coordination bonds with tryptophan and cysteine residues, so these two fillers can also be used to purify proteins without tags but containing these amino acid residues, such as human apoferritin.

Product features and technical indicators

Product Name	IMAC NUPharose FF
Catalog No.	NRPB76L, NRPB76S (pre-packed column)
Matrix	Cross-linked agarose, 6%
Ligand	Nitrilotriacetic acid, precharged with ~17 μmol Ni²⁺/ml
Particle size range^a	45~165 μm
Particle size, d50	~90 μm
Dynamic binding capacity, DBC_10%^b	≥40 mg His-tag protein/mL
Recommended operating flow velocity	60~300 cm/h
Maximum flow velocity^c	1200 cm/h, 0.3 MPa
pH stability	3~12 (recommended operating, pH), 3~12 (long-term), 2~14 (short-term)^d
Chemical stability	Stable to common aqueous buffers: 1 M NaOH, 8 M urea, 6 M guanidine hydrochloride, 70% ethanol. The effects of common reducing agents, chelating agents, detergents, denaturants, etc. on resins are shown in the table below.
Storage & Shipping	20% ethanol，2 ℃~30 ℃
Shelf life	5 years

Key words:

IMAC NUPharose FF

Classification:

Affinity purification

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Sales Phone：0571-82872376

Business Phone：0571-82872986

E-mail：marketing@nuptec.com