NRPB33L

Product Name:

Butyl NUPharose FF

Butyl NUPharose FF is a strong hydrophobic hydrophobic chromatographic separation medium formed by bonding n-butyl to agarose gel microspheres with stable ether bonds. NUPharose is a classic agarose base microsphere developed by NUPTEC. Butyl NUPharose HP is a high-resolution butyl filler, HP is an abbreviation of High Performance. Butyl NUPharose HP has the characteristics of high resolution and is mostly used in the pure stage. The n-butyl "pure hydrophobic" group, as compared to the phenyl group, has a difference in selectivity for different hydrophobic amino acid residues. The product retains the excellent hydrophilicity and large mesh structure of agarose, and has good compatibility with biologically active macromolecules, with high load, less non-specific adsorption, fast flow rate and so on. It is widely used in the laboratory-scale preparation of biological macromolecules such as proteins and peptides with certain hydrophobic properties and the industrial preparation of biopharmaceuticals and bioengineering.

Product features and technical indicators

Product Name	Butyl NUPharose FF	Butyl NUPharose HP
Catalog No.	NRPB33L, NRPB33S (pre-installed column)	NRPB140L, NRPB140S (pre-installed column)
matrix	4% cross-linked agarose	4% highly cross-linked agarose
ligand	n-butyl, ~ 40 μmol/mL	n-butyl, ~ 45 μmol/mL
Particle size range^a	45 ~ 165 μm	20 ~ 60 μm
average particle size	~ 90 μm	~ 35 μm
Dynamic binding load^b	≥25 mg bsa/ml	≥30 mg bsa/ml
recommend working flow rate	150~300 cm/h	60~150
Maximum flow rate and pressure^c	900 cm/h，0.3 MPa	300 cm/h，0.3 MPa
Use of pH	3~13 (long-term operation process),2~14 (short-term operation process such as CIP)
chemical stability	It is stable in the following solutions: commonly used aqueous buffer, 1 mol/L sodium hydroxide, 8 mol/L urea, 6 mol/L guanidine hydrochloride, 70% ethanol, etc.
Storage and transportation	20% ethanol, 2~30 ℃

Key words:

Butyl NUPharose FF

Classification:

Hydrophobic interaction

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E-mail: marketing@nuptec.com

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NUPTEC Inc. Ltd.

Sales Phone:0571-82872376
Business Phone:0571-82872986
E-mail:marketing@nuptec.com
Headquarters Address: Building 25, Qianwan Zhiqi, Jiangnan Science and Technology City, Hangzhou City, Zhejiang Province
Production Base of Li: No.63, No.5 Road, Dangyuan, Li Town, Xiao District, Hangzhou City, Zhejiang Province
Linjiang Production Base: 698 Jing Lu, Linjiang Street, Qiantang District, Hangzhou City, Zhejiang Province

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Sales Phone：0571-82872376

Business Phone：0571-82872986

E-mail：marketing@nuptec.com