NRPB22L

Product Name:

Q NUPharose FF

Q NUPharose series is a strong anion Ion exchange filler, Q is a quaternary ammonium group, and the NUPharose is a NUPTEC biological classic agarose gel chromatography medium-based sphere. Compared with other Q fillers on the market, this product has the characteristics of less non-specific adsorption and less dynamic binding load affected by residence time. It is widely used in the capture, moderate purification and pure purification of biological macromolecules. According to the resolution and application of different occasions, Q NUPharose series can be divided into Q NUPharose FF(~ 90μm), Q NUPharose HP(~ 35μm), Q NUPharose BB(~ 200 μm) three. FF is an abbreviation of Fast Flow. Q NUPharose FF has the characteristics of fast flow rate, which can be used for rapid purification of proteins, with wide applicability and easy amplification. HP is the abbreviation of High Performance. Q NUPharose HP has the characteristics of high resolution and is mostly used in the pure stage. BB is the abbreviation of Big Beads. Q NUPharose BB has a large particle size and is suitable for high viscosity systems, such as blood products, and can also be used for batch static operation.

Product features and technical indicators

Product Name	Q NUPharose FF
Catalog No.	NRPB22L,NRPB22S (Pre-packed Column)
Matrix	6% Cross-linked agarose
Ligand	Quaternary ammonium salt, -CH₂CH(OH)CH₂N(CH₃)₃⁺
Particle size range^a	45~165 μm
Particle size, d₅₀	~90 μm
Ionic capacity	110~160 μmol OH^- /mL
Dynamic binding capacity, DBC_10%	≥40 mg/mL^b ≥110 mg/mL^c
Recommended operating flow velocity	150~300 cm/h
Maximum flow velocity^c	1200 cm/h
pH stability	3~12 (Recommended operating, pH), 3~12 (Long-term)；2~14 (CIP)
Chemical stability	Stable to common aqueous buffers, 1.0 M NaOH, 8 M Urea,6 M guanidine hydrochloride, 30% isopropanol and 70% ethano
Storage & Shipping	20% ethanol, 2~30 ℃
Shelf life	5 years

Key words:

Q NUPharose FF

Classification:

Ion exchange

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NUPTEC Inc. Ltd.

Sales Phone:0571-82872376
Business Phone:0571-82872986
E-mail:marketing@nuptec.com
Headquarters Address: Building 25, Qianwan Zhigu, Jiangnan Science and Technology City, Hangzhou City, Zhejiang Province
Guali Production Base: No. 63, Yuanwu Road, Dangshan Industrial Park, Guali Town, Xiaoshan District, Hangzhou City, Zhejiang Province
Linjiang Production Base: 698 Jing Lu, Linjiang Street, Qiantang District, Hangzhou City, Zhejiang Province

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Sales Phone：0571-82872376

Business Phone：0571-82872986

E-mail：marketing@nuptec.com