NUPTEC Inc. Ltd.

Product Details

Professional Manufaturer of Resins for Protein Purification

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NuPerley Q
NuPerley Q
NuPerley Q
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  • NuPerley Q
  • NuPerley Q
  • NuPerley Q

Catalog No:

NRPB93L

Product Name:

NuPerley Q


NuPerley Q is an ultra-high loading strong anion exchange chromatography medium, which is composed of quaternary ammonium groups (Q) bonded to dextran-grafted high-rigidity sepharose microspheres. This product benefits from two new technology platforms of NUPTEC biology: a new generation of high rigidity agarose microsphere NuPerley and a unique dextran grafting process. The former allows the packing to withstand flow rates and pressures of 3000 cm/h and more than 0.5 MPa, and the latter allows the packing to have an ultra-high loading and is not affected by residence time. For example, at 2 min residence time, the dynamic binding capacity of NuPerley Q to BSA is more than 220 mg/mL, which is 6 times that of traditional Q NUPharose FF.

Product features and technical indicators

Product Name

NuPerley Q

Catalog No.

NRPB93L, NRPB93S (pre-installed column)

matrix

Dextran-grafted high rigidity agarose microspheres

functional group

-CH2CH(OH)CH2N(CH3)3

average particle size

~ 90 μm

Ion exchange容量

110~150 μmol OH-/mL

Dynamic binding loada

220 mg/mL

recommend working flow rate

150~700 cm/h

Maximum flow rateb

3000 cm/h

chemical stability

It is stable in the following solutions: common aqueous buffer, 1 M sodium hydroxide, 8 M urine, 6 M guanidine hydrochloride, 70% ethanol, 30% isopropanol, etc.

pH stability

3~12 (recommend working pH),3~12 (long-term stability);2~14 (short-term stability)

Storage and transportation

20% ethanol, 2 ℃ ~ 30 ℃

Shelf life

5 years