NRPB02L

Product Name:

ProG NUPharose FF affinity filler

ProG NUPharose FF is an antibody affinity filler. Its ligand is recombinant streptococcal protein G(NRPA06S) developed by NUPTEC. The base ball NUPharose is NUPTEC's classic agarose gel base ball. FF is the abbreviation of Fast Flow, which means that the filler can work at high flow rate. The recombinant Streptococcus protein G ligand was expressed by E. coli without animal origin. ProtG NUPharose FF's base ball and ligand are formed by single-point coupling, and with genetic engineering, its dynamic binding load for human IgG is higher than 40 mg/mL, which has great advantages over similar products on the market. Compared to ProA NUPharose FF, ProG NUPharose FF has the advantage of binding to all IgG subtypes, both human and mouse. ProG NUPharose FF has affinity for various types and subtypes of antibodies from human, mouse, rat, rabbit, cow, etc. It can isolate and purify antibodies of different subtypes of various mammals or genetically engineered recombinant proteins containing antibody Fc fragments from ascites, serum, cell culture supernatant or cell extracts, which can meet the needs of research or production on different scales and can also be used for immunoprecipitation.

Product features and technical indicators

Product Name	ProG NUPharose FF
Catalog No.	NRPB02L, NRPB02S (pre-installed column)
matrix	cross-linked agarose，4%
ligand	Recombinant Protein G, ≥6 mg/mL
Particle size range^a	45 ~ 165 μm
average particle size	~ 90 μm
Dynamic binding load^b	≥40 mg h-IgG/mL
recommend working flow rate^c	60~300 cm/h
Maximum flow rate and pressure^d	900 cm/h，0.3 MPa
Use of pH	3~9 (recommend working pH),2~10 (short-term )
chemical stability	Stable to common aqueous buffers: 10~100 mmol/L NaOH (CIP), 6 M guanidine hydrochloride, 70% ethanol (CIP)
Storage and transportation	20% ethanol, 2~8 ℃,shipping 2~30 ℃
Shelf life	3 years

Note: ^aMore than 90% of the microspheres by volume are in this size range; ^bDynamic binding capacity at 10% breakthrough for h-IgG with 6 min residence time; ^cThe flow rate can meet the use conditions of 2-6 min residence time for various types of columns; ^d1 cm diameter column and 10 cm bed height using buffers with the same viscosity as water at 20 °C.

Key words:

ProG NUPharose FF affinity filler

Classification:

Affinity purification

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Tel: 0571-82872376

E-mail: marketing@nuptec.com

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NUPTEC Inc. Ltd.

Sales Phone:0571-82872376
Business Phone:0571-82872986
E-mail:marketing@nuptec.com
Headquarters Address: Building 25, Qianwan Zhigu, Jiangnan Science and Technology City, Hangzhou City, Zhejiang Province
Guali Production Base: No. 63, Yuanwu Road, Dangshan Industrial Park, Guali Town, Xiaoshan District, Hangzhou City, Zhejiang Province
Linjiang Production Base: 698 Jing Lu, Linjiang Street, Qiantang District, Hangzhou City, Zhejiang Province

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Sales Phone：0571-82872376

Business Phone：0571-82872986

E-mail：marketing@nuptec.com