NRPB02L

Product Name:

ProG NUPharose FF affinity filler

ProG NUPharose FF is an antibody affinity filler. Its ligand is recombinant streptococcal protein G(NRPA06S) developed by NUPTEC. The base ball NUPharose is NUPTEC's classic agarose gel base ball. FF is the abbreviation of Fast Flow, which means that the filler can work at high flow rate. The recombinant Streptococcus protein G ligand was expressed by E. coli without animal origin. ProtG NUPharose FF's base ball and ligand are formed by single-point coupling, and with genetic engineering, its dynamic binding load for human IgG is higher than 40 mg/mL, which has great advantages over similar products on the market. Compared to ProA NUPharose FF, ProG NUPharose FF has the advantage of binding to all IgG subtypes, both human and mouse. ProG NUPharose FF has affinity for various types and subtypes of antibodies from human, mouse, rat, rabbit, cow, etc. It can isolate and purify antibodies of different subtypes of various mammals or genetically engineered recombinant proteins containing antibody Fc fragments from ascites, serum, cell culture supernatant or cell extracts, which can meet the needs of research or production on different scales and can also be used for immunoprecipitation.

Product features and technical indicators

Product Name	ProG NUPharose FF
Catalog No.	NRPB02L, NRPB02S (pre-installed column)
matrix	4% cross-linked agarose
ligand	Recombinant streptococcal protein G,≥ 6 mg/mL, single point coupling
Particle size range^a	45 ~ 165 μm
average particle size	~ 90 μm
Dynamic binding load^b	≥40 mg H-igg/ml
recommend working flow rate^c	60~300 cm/h
Maximum flow rate and pressure^d	900 cm/h，0.3 MPa
Use of pH	3~9 (recommend working pH),2~10 (short-term stability)
chemical stability	Stable in the following solutions: common aqueous buffer, 6 mol/L guanidine hydrochloride, 70% ethanol (CIP).
Storage and transportation	20% ethanol, 2~8 ℃ storage, 2~30 ℃ transportation
Shelf life	3 years

Note:^aMore than 90% of the volume of microspheres in this particle size range;^bDBC10% test conditions: 10% flow through, 6 min residence time;^cThe recommend flow rate means that the flow rate can meet the use conditions of 2~6 min residence time under most columns, not only within the flow rate range;^dMaximum test flow rate at 10 cm column height.

Key words:

ProG NUPharose FF affinity filler

Classification:

Affinity purification

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E-mail: marketing@nuptec.com

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NUPTEC Inc. Ltd.

Sales Phone:0571-82872376
Business Phone:0571-82872986
E-mail:marketing@nuptec.com
Headquarters Address: Building 25, Qianwan Zhiqi, Jiangnan Science and Technology City, Hangzhou City, Zhejiang Province
Production Base of Li: No.63, No.5 Road, Dangyuan, Li Town, Xiao District, Hangzhou City, Zhejiang Province
Linjiang Production Base: 698 Jing Lu, Linjiang Street, Qiantang District, Hangzhou City, Zhejiang Province

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Sales Phone：0571-82872376

Business Phone：0571-82872986

E-mail：marketing@nuptec.com