NRPB58L

Product Name:

Ni-TED NUPharose FF

Ni-TED NUPharose FF is a metal chelating affinity filler with the lowest drop-off rate of metal ions. The ligand is tricarboxymethyl ethylenediamine (tris-carboxymethyl ethylene diamine,TED) group, the coordination metal ion is Ni2, the NUPharose is a classic agarose gel basic microsphere developed by NUPTEC biology, and FF is the abbreviation of Fast Flow. The TED ligand of this product has good stability in the five-coordination structure formed by Ni2, and has excellent tolerance to chelating agents, reducing agents, acids, alkalis and other reagents. It can be used normally under the conditions of 10 mM EDTA, 100 mM β-ME or 50 mM DTT, and can maintain excellent chromatographic performance after being treated for 24 h under the condition of pH = 1 or pH = 14, also for long-term storage in 10 mM NaOH, the concentration of imidazole required in the eluent is lower than that of other metal chelating fillers.

Product features and technical indicators

Product Name	Ni-TED NUPharose FF
Catalog No.	NRPB58L, NRPB58S (pre-packed column)
Matrix	Cross-linked agarose, 6%
Ligand	Triscarboxymethylethylenediamine, precharged with ~60 μmol Ni²⁺/mL
Particle size range^a	45~165 μm
Particle size, d50	~90 μm
Dynamic binding capacity, DBC_10%^b	≥20 mg His-tag protein/mL
Recommended operating flow velocity^c	60~300 cm/h
Maximum flow velocity^d	1200 cm/h, 0.3 MPa
pH stability	3~12 (recommended operating, pH), 3~12 (long-term), 2~14 (short-term)
Chemical stability	1) Purification buffer: commonly used aqueous buffer, which may contain 10 mM EDTA, 100 mM β-ME or 50 mM DTT. 2) Cleaning in place (CIP) treatment: 1 M NaOH, 1~2 M NaCl, 70% ethanol, 30% isopropanol, 0.1~0.5% non-ionic surfactant. 3) The performance of the resin remains stable after treatment in the following solutions for a specific time: 10 mM NaOH (long-term), 0.1 M hydrochloric acid (24 h), 6 M guanidine hydrochloride (24 h).
Storage & Shipping	20% ethanol，2 ℃~30 ℃
Shelf life	5 years

Key words:

Ni-TED NUPharose FF

Classification:

Affinity purification

Download Product Description

Online Preview

Tel: 0571-82872376

E-mail: marketing@nuptec.com

Alibaba official website mall

Get Quote

COA Download

Apply for a trial outfit

COOKIES

Our website uses cookies and similar technologies to personalize the advertising shown to you and to help you get the best experience on our website. For more information, see our Privacy & Cookie Policy

COOKIES

required

These cookies are necessary for basic functions such as payment. Standard cookies cannot be turned off and do not store any of your information.

analyze

These cookies collect information, such as how many people are using our site or which pages are popular, to help us improve the customer experience. Turning these cookies off will mean we can't collect information to improve your experience.

Feature

These cookies enable the website to provide enhanced functionality and personalization. They may be set by us or by third-party providers whose services we have added to our pages. If you do not allow these cookies, some or all of these services may not function properly.

advertise

These cookies help us understand what you are interested in so that we can show you relevant advertising on other websites. Turning these cookies off will mean we are unable to show you any personalized advertising.

NUPTEC Inc. Ltd.

Sales Phone:0571-82872376
Business Phone:0571-82872986
E-mail:marketing@nuptec.com
Headquarters Address: Building 25, Qianwan Zhigu, Jiangnan Science and Technology City, Hangzhou City, Zhejiang Province
Guali Production Base: No. 63, Yuanwu Road, Dangshan Industrial Park, Guali Town, Xiaoshan District, Hangzhou City, Zhejiang Province
Linjiang Production Base: 698 Jing Lu, Linjiang Street, Qiantang District, Hangzhou City, Zhejiang Province

Online Message →

Sales Phone：0571-82872376

Business Phone：0571-82872986

E-mail：marketing@nuptec.com