NRPB01L

Product Name:

ProA NUPharose FF antibody affinity filler

ProA NUPharose FF is an antibody affinity filler. Its ligand rProtein A is recombinant Staphylococcus aureus protein A(NRPA04S) developed by NUPTEC. The base ball NUPharose is NUPTEC's classic agarose gel base ball. FF is the abbreviation of Fast Flow, which means that the filler can work at high flow rate. Recombinant Staphylococcus aureus protein A ligand is expressed by E. coli, does not contain animal sources, and retains the wild-type 5 binding domains. Therefore, the loading of ProA NUPharose FF is much higher than that of wild-type protein A filler, and the dynamic binding loading of human IgG is higher than 40 mg/mL. ProA NUPharose FF has affinity for various types and subtypes of antibodies from human, mouse, rat, rabbit, cow, etc. It can isolate and purify various mammalian subtypes of antibodies or genetically engineered recombinant proteins containing antibody Fc fragments from ascites, serum, cell culture supernatant or cell extracts, which can meet the needs of research or production on different scales.

Product features and technical indicators

Product Name	ProA NUPharose FF
Catalog No.	NRPB01L, NRPB01S (pre-installed column)
matrix	4% cross-linked agarose
ligand	Recombinant Staphylococcus aureus protein A,≥ 6 mg/mL
Particle size range^a	45 ~ 165 μm
average particle size	~ 90 μm
Dynamic binding load^b	≥40 mg H-igg/ml
recommend working flow rate^c	60~300 cm/h
Maximum flow rate and pressure^d	900 cm/h，0.3 MPa
Use of pH	3~10 (recommend working pH),3~12 (short-term stability)
chemical stability	It is stable in the following solutions: common aqueous buffer, 10~100 mmol/L NaOH(CIP), 6 mol/L guanidine hydrochloride, 70% ethanol (CIP).
Storage and transportation	20% ethanol, 2~8 ℃ storage, 2~30 ℃ transportation
Shelf life	3 years

Note:^aMore than 90% of the volume of microspheres in this particle size range;^bDBC10% test conditions: 10% flow through, 6 min residence time;^cThe recommended flow rate means that the flow rate can meet the use conditions of 2~6 min residence time under most columns, not only within the flow rate range;^dMaximum test flow rate at 10 cm column height.

Key words:

ProA NUPharose FF antibody affinity filler

Classification:

Affinity purification

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E-mail: marketing@nuptec.com

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NUPTEC Inc. Ltd.

Sales Phone:0571-82872376
Business Phone:0571-82872986
E-mail:marketing@nuptec.com
Headquarters Address: Building 25, Qianwan Zhiqi, Jiangnan Science and Technology City, Hangzhou City, Zhejiang Province
Production Base of Li: No.63, No.5 Road, Dangyuan, Li Town, Xiao District, Hangzhou City, Zhejiang Province
Linjiang Production Base: 698 Jing Lu, Linjiang Street, Qiantang District, Hangzhou City, Zhejiang Province

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Sales Phone：0571-82872376

Business Phone：0571-82872986

E-mail：marketing@nuptec.com