NUPTEC Inc. Ltd.

Product Details

Professional Manufaturer of Resins for Protein Purification

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ProA NUPharose FF antibody affinity filler
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  • ProA NUPharose FF antibody affinity filler

Catalog No:

NRPB01L

Product Name:

ProA NUPharose FF antibody affinity filler



Product features and technical indicators

Product Name

ProA NUPharose FF

Catalog No.

NRPB01L, NRPB01S (pre-installed column)

matrix

Cross-linked agarose, 4%

ligand

Recombinant protein A (E.coli), ≥6 mg/mL

Particle size rangea

45 ~ 165 μm

average particle size

~ 90 μm

Dynamic binding loadb

≥40 mg h-IgG/mL

recommend working flow ratec

60~300 cm/h

Maximum flow rate and pressured

900 cm/h,0.3 MPa

Use of pH

3~10 (recommended operating pH), 3~12 (short-term)

chemical stability

Stable to common aqueous buffers: 10~100 mmol/L NaOH(CIP), 6 M guanidine hydrochloride, 70% ethanol (CIP)

Storage and transportation

20% ethanol, storage 2~8 ℃, shipping 2~30 ℃

Shelf life

3 years
Note: aMore than 90% of the microspheres by volume are in this size range; bDynamic binding capacity at 10% breakthrough for h-IgG with 6 min residence time; cThe flow rate can meet the use conditions of 2-6 min residence time for various types of columns; d1 cm diameter column and 10 cm bed height using buffers with the same viscosity as water at 20℃.