Product Details
Professional Manufaturer of Resins for Protein Purification
Catalog No:
NRPB01L
Product Name:
ProA NUPharose FF antibody affinity filler
Product features and technical indicators
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Product Name |
ProA NUPharose FF |
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Catalog No. |
NRPB01L, NRPB01S (pre-installed column) |
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matrix |
Cross-linked agarose, 4%
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ligand |
Recombinant protein A (E.coli), ≥6 mg/mL
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Particle size rangea |
45 ~ 165 μm |
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average particle size |
~ 90 μm |
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Dynamic binding loadb |
≥40 mg h-IgG/mL
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recommend working flow ratec |
60~300 cm/h |
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Maximum flow rate and pressured |
900 cm/h,0.3 MPa |
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Use of pH |
3~10 (recommended operating pH), 3~12 (short-term)
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chemical stability |
Stable to common aqueous buffers: 10~100 mmol/L NaOH(CIP), 6 M guanidine hydrochloride, 70% ethanol (CIP)
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Storage and transportation |
20% ethanol, storage 2~8 ℃, shipping 2~30 ℃
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Shelf life |
3 years |
Note: aMore than 90% of the microspheres by volume are in this size range; bDynamic binding capacity at 10% breakthrough for h-IgG with 6 min residence time; cThe flow rate can meet the use conditions of 2-6 min residence time for various types of columns; d1 cm diameter column and 10 cm bed height using buffers with the same viscosity as water at 20℃.
Key words:
ProA NUPharose FF antibody affinity filler
Classification:
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