NUPTEC Inc. Ltd.

Product Details

Professional Manufaturer of Resins for Protein Purification

%{tishi_zhanwei}%
NuPerley Core 700
NuPerley Core 700
NuPerley Core 700
+
  • NuPerley Core 700
  • NuPerley Core 700
  • NuPerley Core 700

Catalog No:

NRPB145L

Product Name:

NuPerley Core 700


NuPerley Core 700 is a multimodal filler with a core-shell double-layer structure. Its inert shell thickness is ~ 5μm, which plays the role of Gel filtration. Its exclusion limit for globulin is ~ 700 kDa, and target molecules cannot enter the inside of the microsphere and flow through during purification. Its inner core ligand is n-octylamine, which is hydrophobic and positively charged. It uses multimodal to bind as many non-target molecules as possible to make the operation window larger. NuPerley Core 700 is widely used in the field of purification of larger biomolecules, such as exosomes, plasmid DNA, and virus particles. Compared with traditional Gel filtration fillers, the throughput of NuPerley Core 700 is improved by more than an order of magnitude.

Product features and technical indicators

Product Name NuPerley Core 700

Catalog No.

NRPB145L, NRPB145S (pre-installed column)

matrix

high rigidity agarose

average particle size

~ 90 μm, inert shell thickness ~ 5 μm

ligand

n-octylamine

ligand density

≥40µmol/mL

Dynamic binding loada

≥10 mg ovalbumin/mL

recommend working flow rate

150~700 cm/h

Maximum flow rate and pressure resistanceb

1500 cm/h,0.5 MPa

chemical stability

It is stable in the following solutions: common aqueous buffer, 1 M sodium hydroxide, 8 M urine, 6 M guanidine hydrochloride, 70% ethanol, 30% isopropanol, etc.

pH stability

3~12 (recommend working pH),3~12 (long-term stability);2~14 (short-term stability)

Storage and transportation

20% ethanol, 2~30 oC

Shelf life

5 years