NUPTEC Inc. Ltd.

Product Details

Professional Manufaturer of Resins for Protein Purification

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NuPerley S
NuPerley S
NuPerley S
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  • NuPerley S
  • NuPerley S
  • NuPerley S

Catalog No:

NRPB108L

Product Name:

NuPerley S


NuPerley S is an ultra-high loading of ion exchange chromatography medium, by the sulfonic acid methyl ester (S) and dextran grafted high rigid agarose gel microspheres. This product benefits from two new technology platforms of NUPTEC biology: a new generation of high rigidity agarose microsphere NuPerley and a unique dextran grafting process. The former allows the packing to withstand flow rates and pressures of 3000 cm/h and more than 0.5 MPa, and the latter allows the packing to have an ultra-high loading and is not affected by residence time. For example, the dynamic binding load of NuPerley S to lysozyme was greater than 170 mg/mL at a residence time of 2 min. Compared to other SP fillers on the market, this product has no risk of allyl residues.

Product features and technical indicators

Product Name

NuPerley S

Catalog No.

NRPB108L, NRPB108S (pre-installed column)

matrix

Dextran-grafted high rigidity agarose microspheres

functional group

methyl sulfonate

average particle size

~ 90 μm

Ion exchange容量

80~120 μmol H/mL

Dynamic binding loada

170 mg/mL

recommend working flow rate

150~700 cm/h

Maximum flow ratec

3000 cm/h

chemical stability

It is stable in the following solutions: common aqueous buffer, 1 M sodium hydroxide, 8 M urea, 6 M guanidine hydrochloride, 70% ethanol, etc.

pH stability

4~12 (working pH);3~12 (long-term stability);2~14 (short-term stability)

Storage and transportation

20% ethanol, 2 ℃ ~ 30 ℃

Shelf life

5 years