NRPB104L

Product Name:

NuPerley DEAE HR

NuPerley DEAE HR is a strong anion exchange chromatography medium with high resolution and high flow rate, which is composed of diethylaminoethyl (DEAE) and a new generation of small particle size, high rigidity agarose gel microspheres NuPerley bonded. The average particle size of this product is 35 μm, and the flow rate of more than 900 cm/h can still be achieved. Thanks to the optimization of Ion exchange capacity (ligand density), the dynamic binding load of NuPerley DEAE HR to BSA is more than 60 mg/mL at a residence time of 2 min, which is NUPharose with traditional DEAE FF and is mainly used for moderate purification and fine purification. When the purification scale is not very large, or the initial sample is relatively clean (such as supernatant expression and high expression of samples), this product can also be used to achieve high purity through one-step purification. Based on these characteristics, NuPerley DEAE HR can replace and upgrade DEAE NUPharose FF in many fields. Weak anion exchange(Q) fillers have a different selectivity than strong anion exchange(Q) fillers, and DEAE fillers can be considered when Q fillers do not meet the selectivity requirements.

Product features and technical indicators

Product Name	NuPerley DEAE HR
Catalog No.	NRPB104L, NRPB104S (pre-installed column)
matrix	High resolution, high rigidity agarose microspheres
functional group	Diethylaminoethyl,-CH2CH2NH(CH2CH3)2
average particle size	~ 35 μm
Ion exchange容量	60~100 μmol OH-/mL
Dynamic binding load^a	≥60 mg/mL
recommend working flow rate	50~150 cm/h
Maximum flow rate^b	900 cm/h
chemical stability	It is stable in the following solutions: common aqueous buffer, 1 M sodium hydroxide, 8 M urea, 6 M guanidine hydrochloride, 70% ethanol, 30% isopropanol, etc.
pH stability	3~9 (recommend working pH),3~12 (long-term stability);2~14 (short-term stability)
Storage and transportation	20% ethanol, 2~30 ℃
Shelf life	5 years

Key words:

NuPerley DEAE HR

Classification:

Ion exchange

Download Product Description

Online Preview

Tel: 0571-82872376

E-mail: marketing@nuptec.com

Alibaba official website mall

Get Quote

COA Download

Apply for a trial outfit

COOKIES

Our website uses cookies and similar technologies to personalize the advertising shown to you and to help you get the best experience on our website. For more information, see our Privacy & Cookie Policy

COOKIES

required

These cookies are necessary for basic functions such as payment. Standard cookies cannot be turned off and do not store any of your information.

analyze

These cookies collect information, such as how many people are using our site or which pages are popular, to help us improve the customer experience. Turning these cookies off will mean we can't collect information to improve your experience.

Feature

These cookies enable the website to provide enhanced functionality and personalization. They may be set by us or by third-party providers whose services we have added to our pages. If you do not allow these cookies, some or all of these services may not function properly.

advertise

These cookies help us understand what you are interested in so that we can show you relevant advertising on other websites. Turning these cookies off will mean we are unable to show you any personalized advertising.

NUPTEC Inc. Ltd.

Sales Phone:0571-82872376
Business Phone:0571-82872986
E-mail:marketing@nuptec.com
Headquarters Address: Building 25, Qianwan Zhigu, Jiangnan Science and Technology City, Hangzhou City, Zhejiang Province
Production Base of Li: No.63, No.5 Road, Dangyuan, Li Town, Xiao District, Hangzhou City, Zhejiang Province
Linjiang Production Base: 698 Jing Lu, Linjiang Street, Qiantang District, Hangzhou City, Zhejiang Province

Online Message →

Sales Phone：0571-82872376

Business Phone：0571-82872986

E-mail：marketing@nuptec.com