NUPTEC Inc. Ltd.

Product Details

Professional Manufaturer of Resins for Protein Purification

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NuPerley CM
NuPerley CM
NuPerley CM
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  • NuPerley CM
  • NuPerley CM
  • NuPerley CM

Catalog No:

NRPB91L

Product Name:

NuPerley CM


NuPerley CM is a high loading weak cation ion exchange chromatography medium, which is composed of carboxymethyl group (CM) bonded to dextran grafted high rigid agarose gel microspheres. This product benefits from two new technology platforms of NUPTEC biology: a new generation of high rigidity agarose microsphere NuPerley and a unique dextran grafting process. The former allows the packing to withstand flow rates and pressures of 3000 cm/h and more than 0.5 MPa, and the latter allows the packing to have an ultra-high loading and is not affected by residence time. For example, at 2 min residence time, the dynamic binding load of NuPerley CM to lysozyme is more than 140 mg/mL.

Product features and technical indicators

Product Name

NuPerley CM

Catalog No.

NRPB91L, NRPB91S (pre-installed column)

matrix

Dextran-grafted high rigidity agarose microspheres

functional group

Carboxymethyl,-CH2COO-

average particle size

~ 90 μm

Ion exchange容量

90~130 μmol H/mL

Dynamic binding loada

140 mg/mL

recommend working flow rate

150~700 cm/h

Maximum flow rateb

3000 cm/h

chemical stability

It is stable in the following solutions: common aqueous buffer, 1 M sodium hydroxide, 8 M urea, 6 M guanidine hydrochloride, 70% ethanol, etc.

pH stability

6~10 (working pH);3~12 (long-term stability);2~14 (short-term stability)

Storage and transportation

20% ethanol, 2 ℃ ~ 30 ℃

Shelf life

5 years