NRPB107L

Product Name:

NuPerley SP HR

NuPerley SP HR is a strong anion exchange chromatography medium with high resolution and high flow rate. It is NuPerley bonded by sulfopropyl (SP) and a new generation of small particle size and high rigidity agarose gel microspheres. The average particle size of this product is 35 μm, and the flow rate of more than 900 cm/h can still be achieved. Thanks to the optimization of Ion exchange capacity (ligand density), the dynamic binding load of NuPerley SP HR to lysozyme is more than 90 mg/mL at a residence time of 2 min. Compared with other SP fillers on the market, the sulfonation reaction of this product is strictly controlled in the production process, and the residual allyl content is very small. When the purification scale is not very large, or the initial sample is relatively clean (such as supernatant expression and high expression of samples), this product can also be used to achieve high purity through one-step purification.

Product features and technical indicators

Product Name	NuPerley SP HR
Catalog No.	NRPB107L, NRPB107S (pre-installed column)
matrix	High resolution, high rigidity agarose microspheres
functional group	-CH2CH2CH2SO3-
average particle size	~ 35 μm
Ion exchange容量	130~170 μmol H/mL
Dynamic binding load^a	≥90 mg/mL
recommend working flow rate	50~150 cm/h
Maximum flow rate^c	900 cm/h
chemical stability	It is stable in the following solutions: common aqueous buffer, 1 M sodium hydroxide, 8 M urea, 6 M guanidine hydrochloride, 70% ethanol, etc.
pH stability	4~12 (working pH);3~12 (long-term stability);2~14 (short-term stability)
Storage and transportation	20% ethanol, 2 ℃ ~ 30 ℃
Shelf life	5 years

Key words:

NuPerley SP HR

Classification:

Ion exchange

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Tel: 0571-82872376

E-mail: marketing@nuptec.com

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NUPTEC Inc. Ltd.

Sales Phone:0571-82872376
Business Phone:0571-82872986
E-mail:marketing@nuptec.com
Headquarters Address: Building 25, Qianwan Zhigu, Jiangnan Science and Technology City, Hangzhou City, Zhejiang Province
Production Base of Li: No.63, No.5 Road, Dangyuan, Li Town, Xiao District, Hangzhou City, Zhejiang Province
Linjiang Production Base: 698 Jing Lu, Linjiang Street, Qiantang District, Hangzhou City, Zhejiang Province

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Sales Phone：0571-82872376

Business Phone：0571-82872986

E-mail：marketing@nuptec.com