NUPTEC Inc. Ltd.

Product Details

Professional Manufaturer of Resins for Protein Purification

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NuPerley CM HR
NuPerley CM HR
NuPerley CM HR
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  • NuPerley CM HR
  • NuPerley CM HR
  • NuPerley CM HR

Catalog No:

NRPB92L

Product Name:

NuPerley CM HR


NuPerley CM HR is a strong anion exchange chromatography medium with high resolution and high flow rate. It is NuPerley bonded by carboxymethyl (CM) and a new generation of small particle size and high rigidity agarose gel microspheres. The average particle size of this product is 35 μm, and the flow rate of more than 900 cm/h can still be achieved. Thanks to the optimization of Ion exchange capacity (ligand density), the dynamic binding load of NuPerley CM HR to lysozyme is more than 60 mg/mL at a residence time of 2 min. When the purification scale is not very large, or the initial sample is relatively clean (such as supernatant expression and high expression of samples), this product can also be used to achieve high purity through one-step purification.

Product features and technical indicators

Product Name

NuPerley CM HR

Catalog No.

NRPB92L, NRPB92S (pre-installed column)

matrix

High resolution, high rigidity agarose microspheres

functional group

Carboxymethyl,-CH2COO-

average particle size

~ 35 μm

Ion exchange容量

80~140 μmol H/mL

Dynamic binding loada

≥60 mg/mL

recommend working flow rate

50~150 cm/h

Maximum flow rateb

900 cm/h

chemical stability

It is stable in the following solutions: common aqueous buffer, 1 M sodium hydroxide, 8 M urea, 6 M guanidine hydrochloride, 70% ethanol, etc.

pH stability

6~10 (working pH);3~12 (long-term stability);2~14 (short-term stability)

Storage and transportation

20% ethanol, 2~30 ℃

Shelf life

5 years