NRPB102L

Product Name:

DEAE NUPharose HP

DEAE NUPharose series is weakly negative Ion exchange packing, DEAE is diethylaminoethyl, NUPharose is NUPTEC biological classic agarose gel chromatography media based spheres. Weak anion exchange(Q) fillers have a different selectivity than strong anion exchange(Q) fillers, and DEAE fillers can be considered when Q fillers do not meet the selectivity requirements. According to the resolution and application of different occasions, DEAE NUPharose series can be divided into DEAE NUPharose FF(~ 90μm) and DEAE NUPharose HP(~ 35μm) two. FF is the abbreviation of Fast Flow. DEAE NUPharose FF has the characteristics of fast flow rate, which can be used for rapid purification of proteins, with wide applicability and easy amplification. HP is the abbreviation of High Performance. DEAE NUPharose HP has the characteristics of high resolution and is mostly used in the pure stage.

Product features and technical indicators

Product Name	DEAE NUPharose FF	DEAE NUPharose HP
Catalog No.	NRPB21L、 NRPB21S (pre-mounted column)	NRPB102L、 NRPB102S (Preloaded)
matrix	6% cross-linked agarose
functional group	Diethylaminoethyl,-CH2CH2NH(CH2CH3)2
Particle size range^a	45 ~ 165 μm	20 to 50 μm
average particle size	~ 90 μm	~ 35 μm
Ion exchange容量	80~120 μmol OH-/mL	40~80 μmol OH-/mL
Dynamic binding load^b	≥50 mg/mL	≥40 mg/mL
recommend working flow rate	150~300 cm/h	60~150 cm/h
Maximum flow rate^c	1200 cm/h	300 cm/h
chemical stability	It is stable in the following solutions: common aqueous buffer, 1 M sodium hydroxide, 8 M urea, 6 M guanidine hydrochloride, 70% ethanol.
pH stability	3~9 (recommend working pH),3~12 (long-term stability);2~14 (short-term stability)
Storage and transportation	20% ethanol, 2~30 ℃
Shelf life	5 years

Key words:

DEAE NUPharose HP

Classification:

Ion exchange

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NUPTEC Inc. Ltd.

Sales Phone:0571-82872376
Business Phone:0571-82872986
E-mail:marketing@nuptec.com
Headquarters Address: Building 25, Qianwan Zhiqi, Jiangnan Science and Technology City, Hangzhou City, Zhejiang Province
Production Base of Li: No.63, No.5 Road, Dangyuan, Li Town, Xiao District, Hangzhou City, Zhejiang Province
Linjiang Production Base: 698 Jing Lu, Linjiang Street, Qiantang District, Hangzhou City, Zhejiang Province

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Sales Phone：0571-82872376

Business Phone：0571-82872986

E-mail：marketing@nuptec.com