NRPA06S

Product Name:

NRPA06 Recombinant Streptococcus Protein G(r-SPG)

Streptococcus G protein (Streptococcus Protein G referred to as SPG) is a protein in the cell wall of G and C streptococci, which can specifically bind to the Fc segment of immunoglobulin IgG molecules without affecting the ability of its Fab segment to bind to antigen molecules. It can not only combine with all subclasses of human, mouse and rat IgG, but also with IgG of guinea pig, rabbit, goat, cow, sheep and horse. It is obviously better than Staphylococcus aureus protein A(Staphylococcus Protein A, referred to as SPA) which can only combine with human and mouse IgG. The use of SPG is extremely wide, and can be used for separation and purification or removal of IgG from serum, purification of monoclonal antibodies, separation of antigen-antibody complexes, and the like. After SPG is combined with agarose gel, affinity fillers can be prepared for antibody purification, and different subtypes of IgG can be isolated and purified from serum, ascites, cell culture supernatant, etc. Native SPG has both albumin and cell surface binding domains in addition to the IgG binding domain. Recombinant SPG removes albumin and cell surface binding domain to reduce non-specific binding, it has greater affinity than native SPG and SPA.

Product features and technical indicators

Product Name	Recombinant Streptococcal Protein G(r-SPG)
Catalog No.	NRPA06S
Specifications	1 mg ,10 mg ,100 mg ,500 mg ,1g
Product form	Freeze dried powder; buffer system 5 mM PB(pH 7.4) before lyophilization
molecular weight	28 kDa
isoelectric point	4.79
Source	Escherichia coli
Purity	≥90%
Save condition	-20 ℃ preservation
Validity Period	3 years

Note:

1) Dissolved and placed on the ice bath and try to use immediately;

2) If it is not used up at one time, please prepare the mother liquor before use and sub-pack it into small specifications (to ensure the dosage of one test) and store it at -65 ℃ to avoid repeated freezing and thawing affecting protein activity.

3) avoid the use of strong acids, strong bases, strong oxidants, high concentrations of organic solvents and other reagents that are easy to cause protein denaturation;

4) This product is limited to scientific research use.

Key words:

NRPA06 Recombinant Streptococcus Protein G(r-SPG)

Classification:

Ligand proteins

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