NUPTEC Inc. Ltd.

Product Details

Professional Manufaturer of Resins for Protein Purification

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NuPore 200
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  • NuPore 200

Catalog No:

NRPB87L

Product Name:

NuPore 200


The NuPore series is a high-performance molecular sieve filler formed by bonding dextran to a cross-linked agarose matrix. The average particle size of the composite filler microspheres is about 35 μm, which combines the excellent mechanical properties of agarose and the high selectivity of dextran network. Compared with the traditional molecular sieve filler, NuPore series has the advantages of strong rigidity, high resolution and fast flow rate, which can be used for the separation process of groups such as salt removal and liquid exchange, and can also be used for the separation process of biological macromolecules such as polypeptides, proteins, nucleic acids and natural drugs with similar molecular weights.

Product features and technical indicators

Product Name

NuPore 30

NuPore 75

NuPore 200

Catalog No.

NRPB85

NRPB86

NRPB87

Appearance

Milky White gel microsphere dispersions, lyophilized powders (customized) may also be provided.

Particle size range of filler, μm

The volume ratio of microspheres in the range of 20~50 μm is> 80%

Average particle size of filler, μm

~35

Freeze-dried powder swelling w/wa

5.5±0.5

8.5±0.5

11.5±0.5

Globulin separation range, Da

<1 × 104

3 × 103 to 7 × 104

1 × 104 ~ 6 × 105

Flow rate

The recommend working flow rate is 15~100 cm/h, and the maximum flow rate is> 300 cm/h

pH stability, (operating)

3~12

pH stability,(CIP)

1~14

Resistance to back pressure, MPa

0.3

chemical stability

It is stable in various commonly used buffer systems, such as 6 mol/L guanidine hydrochloride, 1 mol/L acetic acid, 8 mol/L urea, 30% isopropanol; It can be cleaned with 0.5~1 mol/L NaOH in place.

Sterilization

pH = 7, autoclaving at 121 ℃ for 30 min

Storage and transportation

2~30 ℃,20% ethanol