NRPA08S

Product Name:

NRPA08 Recombinant alkali-resistant protein A(rat-PA)

Recombinant alkali-resistant protein A was modified on the basis of natural Staphylococcus aureus protein A. Compared with natural protein A, the binding site with Fab was removed, the specificity of recombinant alkali-resistant protein A and Fc was enhanced, and the protein stability and alkali resistance were improved. After the recombinant alkali-resistant protein A is bonded to the agarose gel, an affinity filler for antibody purification can be prepared, and antibodies of various mammalian subtypes or genetically engineered recombinant proteins containing antibody Fc fragments can be isolated and purified from ascites, serum, cell culture supernatant or cell extracts. A cysteine was added to the C- terminus of recombinant alkali-resistant protein A to enhance its directional covalent bonding with the gel.

Product features and technical indicators

Product Name	重组耐碱蛋白 A （Recombinant Alkali-tolerant Protein A, rat-PA）
Catalog No.	NRPA08S
Specifications	1 mg ,10 mg ,100 mg ,500 mg ,1g
Product form	Lyophilized powder, 5mM PB (4mM Na2HPO4, 1mM NaH2PO4, pH 7.4) before lyophilization
molecular weight	31.2kDa（SDS-PAGE 约 28 kDa）
isoelectric point	5.24
Source	Escherichia coli
Purity	≥95%
Save condition	-20 ℃ preservation
Validity Period	3 years

Note:

1) Dissolved and placed on the ice bath and try to use immediately;

2) If it is not used up at one time, please prepare the mother liquor before use and sub-pack it into small specifications (to ensure the dosage of one test) and store it at -65 ℃ to avoid repeated freezing and thawing affecting protein activity.

3) avoid the use of strong acids, strong bases, strong oxidants, high concentrations of organic solvents and other reagents that are easy to cause protein denaturation;

4) This product is limited to scientific research use.

Key words:

NRPA08 Recombinant alkali-resistant protein A(rat-PA)

Classification:

Ligand proteins

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