Product Details
Professional Manufaturer of Resins for Protein Purification
Catalog No:
NRPB101L
Product Name:
Q NUPharose BB
Q NUPharose series is a strong anion Ion exchange filler, Q is a quaternary ammonium group, and the NUPharose is a NUPTEC biological classic agarose gel chromatography medium-based sphere. Compared with other Q fillers on the market, this product has the characteristics of less non-specific adsorption and less dynamic binding load affected by residence time. It is widely used in the capture, moderate purification and pure purification of biological macromolecules. According to the resolution and application of different occasions, Q NUPharose series can be divided into Q NUPharose FF(~ 90μm), Q NUPharose HP(~ 35μm), Q NUPharose BB(~ 200 μm) three. FF is an abbreviation of Fast Flow. Q NUPharose FF has the characteristics of fast flow rate, which can be used for rapid purification of proteins, with wide applicability and easy amplification. HP is the abbreviation of High Performance. Q NUPharose HP has the characteristics of high resolution and is mostly used in the pure stage. BB is the abbreviation of Big Beads. Q NUPharose BB has a large particle size and is suitable for high viscosity systems, such as blood products, and can also be used for batch static operation.
Product features and technical indicators
Product Name | Q NUPharose BB |
Catalog No. | NRPB101L, NRPB101S (Pre-packed Column) |
Matrix | 6% Cross-linked agarose |
Ligand | Quaternary ammonium salt, -CH2CH(OH)CH2N(CH3)3+ |
Particle size rangea | 100~300 μm |
Particle size, d50 | ~200 μm |
Ionic capacity | 100~150 μmol OH- /mL |
Dynamic binding capacity, DBC10% | ≥50 mg/mLd |
Recommended operating flow velocity | 150~300 cm/h |
Maximum flow velocityc | 1200 cm/h |
pH stability | 3~12 (Recommended operating, pH), 3~12 (Long-term);2~14 (CIP) |
Chemical stability | Stable to common aqueous buffers, 1.0 M NaOH, 8 M Urea,6 M guanidine hydrochloride, 30% isopropanol and 70% ethano |
Storage & Shipping | 20% ethanol, 2~30 ℃ |
Shelf life | 5 years |
Key words:
Q NUPharose BB
Classification:
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