NRPB136L

Product Name:

Con A NUPharose FF

Con A NUPharose FF is a biological Affinity chromatograph separation medium formed by bonding Concanavalin A(Con A) to agarose gel microspheres. It is mainly used for the separation and purification of various glycoproteins (including glycoprotein viruses), polysaccharides and glycolipids with mannitol and glucose residues. Con A is a tetrameric metalloprotein extracted from Concanavala and a type of lectin. In the presence of Mn2 + and Ca2 +, it can bind α-D-mannopyranosyl, α-D-glucopyranosyl and molecules with sterically related residues of C- 3, C- 4 or C- 6 hydroxyl groups. The application fields of Con A NUPharose FF also include the purification of enzyme-antibody conjugates, the purification of IgM, the separation and purification of cell surface glycoproteins in detergent-dissolved membranes, the separation of membrane vesicles, and the study of changes in the composition of carbohydrate-containing substances.

Product features and technical indicators

Product Name	Con A NUPharose FF
Catalog No.	NRPB136L, NRPB136S (pre-installed column)
matrix	cross-linked agarose
ligand	With A,≥ 8 mg With A/mL
Particle size range^a	45-165μm
average particle size	~ 90 μm
combining ability^b	≥30 mg Thyroglobulin/mL
recommend working flow rate^c	15~150 cm/h
Maximum flow rate and pressure^d	900 cm/h, 0.3 MPa
Use of pH	pH 4~9
chemical stability	Stable in the following solutions: commonly used aqueous buffer; should avoid the chelating agent EDTA, 8M urea, or pH below 3 in order to avoid the loss of metal ions in the lectin and loss of activity.
Storage and transportation	20% ethanol, 0.1 M acetate,1 M NaCl, 1 mM CaCl2, 1 mM MnCl2, 1 mM MgCl2,pH 6.0. 2~8 DEG C preservation and transportation.
Shelf life	3 years

Key words:

Con A NUPharose FF

Classification:

Affinity purification

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