NRPB113L

Product Name:

NuPerley Ni-TED

NuPerley Ni-TED is a metal chelating filler with tricarboxymethyl ethylenediamine (tris-carboxymethyl ethylene diamine,TED) group as ligand, the coordination metal ion is Ni2, and the metal ion drop rate is very low. It is NuPerley as a new generation of high rigidity agarose gel basic microspheres developed by NUPTEC biology. The TED ligand of this product has good stability in the five-coordination structure formed by Ni2, and has excellent tolerance to chelating agents, reducing agents, acids, alkalis and other reagents. It can be used normally under the conditions of 10 mM EDTA, 100 mM β-ME or 50 mM DTT, and can maintain excellent chromatographic performance after being treated for 24 h under the condition of pH = 1 or pH = 14, also for long-term storage in 10 mM NaOH, the concentration of imidazole required in the eluent is lower than that of other metal chelating fillers.

Product features and technical indicators

Product Name	NuPerley Ni-TED
Catalog No.	NRPB113L, NRPB113S (pre-installed column)
matrix	6% cross-linked agarose
ligand	Three carboxymethyl ethylenediamine, chelating ~ 30 mol Ni2 /mL.
Particle size range^a	30 to 100 μm
average particle size	~ 60 μm
Dynamic binding load^b	~ 20 mg/mL Histidine-tagged protein
recommend working flow rate	60~300 cm/h
Maximum flow rate and pressure^c	1500 cm/h，0.5 MPa
Use of pH	3~12 (recommend working pH),3~12 (long-term stability);2~14 (short-term stability)
chemical stability	1) Purification buffer: A commonly used aqueous buffer, which can contain 10 mM EDTA,100 mM β-ME or 50 mM DTT. 2) Clean-in-place (CIP) treatment: 1 M NaOH, 1~2 M NaCl, 70% ethanol, 30% isopropanol, 0.1~0.5 non-ionic surfactant. 3) The packing properties remain stable in the following solutions for a specific time: 10 mM NaOH (long term), 0.1 M hydrochloric acid (24 h), 6 M guanidine hydrochloride (24 h).
Storage and transportation	20% ethanol, 2~30 ℃
Shelf life	5 years

Key words:

NuPerley Ni-TED

Classification:

Affinity purification

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NUPTEC Inc. Ltd.

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Sales Phone：0571-82872376

Business Phone：0571-82872986

E-mail：marketing@nuptec.com