NRPB61L

Product Name:

ST2 NUPharose FF

ST2 NUPharose FF is a mutant of streptavidin (ST2 ligand) and NUPTEC bio-NUPharose sepharose coupled to form and chromatographic separation medium for isolation and purification of Strep II or Twin Strep tag proteins. The Strep II tag is a small tag (WSHPQFEK) of 8 amino acids, which generally does not affect the structure and function of the fusion protein, and is commonly used in the detection and purification of fusion expression proteins. Twin Strep tag is a tag of two Strep II in tandem. Compared with Strep II tag, Twin Strep tag has higher affinity with ST2 ligand. The ST2 ligand of this product has a further increased affinity for Strep II tags and a significantly reduced affinity for biotin compared to the previous generation of ST ligands. Therefore, this product can bind the Strep II fusion protein more firmly, and when the fusion protein expression is low, the relative capture amount is higher, and the load and yield are higher. In terms of elution, biotin can be used for elution, which is more economical than the expensive desthiobiotin elution, and can be regenerated with 10-50 mM NaOH after use. This product is highly specific for the Strep II tag and generally requires only one step of purification to obtain highly pure protein samples.

Product features and technical indicators

Product Name	ST2 NUPharose FF
Catalog No.	NRPB61L, NRPB61S (pre-installed column)
matrix	4% cross-linked agarose
ligand	ST2配基，≥5 mg/ml
Particle size range^a	45 ~ 165 μm
average particle size	~ 90 μm
comprehensive ability^b	8~10 mg/ml Strep II Tag Protein
recommend working flow rate	60~150 cm/h
Maximum flow rate and pressure^c	900 cm/h，0.3 MPa
Use of PH	6~10 (recommend working pH),10~50 mM NaOH(CIP cleaning)
chemical stability	It is stable in the following solutions: commonly used aqueous buffer, 8 mol/L urea, etc.
Storage and transportation	20% ethanol, 2~8 ℃ (storage),2~30 ℃ (transportation)
Shelf life	3 years

Key words:

ST2 NUPharose FF

Classification:

Affinity purification

Tel: 0571-82872376

E-mail: marketing@nuptec.com

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NUPTEC Inc. Ltd.

Sales Phone:0571-82872376
Business Phone:0571-82872986
E-mail:marketing@nuptec.com
Headquarters Address: Building 25, Qianwan Zhigu, Jiangnan Science and Technology City, Hangzhou City, Zhejiang Province
Guali Production Base: No. 63, Yuanwu Road, Dangshan Industrial Park, Guali Town, Xiaoshan District, Hangzhou City, Zhejiang Province
Linjiang Production Base: 698 Jing Lu, Linjiang Street, Qiantang District, Hangzhou City, Zhejiang Province

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Sales Phone：0571-82872376

Business Phone：0571-82872986

E-mail：marketing@nuptec.com