NRPB44L

Product Name:

Biotin NUPhrose FF

Biotin NUPharose FF is a Affinity chromatograph separation medium formed by bonding D-biotin to NUPharose agarose-based balls. The biotin ligand in the medium can highly specifically bind to avidin and streptavidin, with very high affinity, and can be used for the immobilization of avidin, streptavidin and their conjugates, and the immobilized products can be applied to diagnostic detection, protein purification, etc. It can also be used directly for the purification of avidin and streptavidin mutants that weaken affinity.

Product features and technical indicators

Product Name	Biotin NUPhrose FF
Catalog No.	NRPB44L, NRPB44S (pre-installed column)
matrix	6% cross-linked agarose
ligand	D-Biotin, ≥ 5 μmol/mL
Particle size range^a	45 ~ 165 μm
average particle size	~ 90 μm
Dynamic binding load^b	~ 30 mg streptavidin mutant/mL medium
recommend working flow rate	60~150 cm/h
Maximum flow rate and pressure^c	900 cm/h，0.3 MPa
Use of PH	3~12 (recommend working pH),3~13(CIP cleaning)
chemical stability	It is stable in the following solutions: commonly used aqueous buffer, 0.1 mol/L NaOH, 8 mol/L urea, 2% Tween 20, etc.
Storage and transportation	20% ethanol, 2~8 ℃ (storage),2~30 ℃ (transportation)
Shelf life	3 years

Key words:

Biotin NUPhrose FF

Classification:

Affinity purification

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Sales Phone：0571-82872376

Business Phone：0571-82872986

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