NUPTEC Inc. Ltd.

Product Details

Professional Manufaturer of Resins for Protein Purification

%{tishi_zhanwei}%
Recombinant human acidic fibroblast growth factor
+
  • Recombinant human acidic fibroblast growth factor

Catalog No:

NRPA15S

Product Name:

Recombinant human acidic fibroblast growth factor


Acidic Fibroblast Factor (Fibroblast Growth Factor-acidic, aFGF), also known as FGF1, belongs to the FGF family and participates in tissue damage repair. It has trophic and mitogenic differentiation effects on a variety of cells derived from mesoderm and neuroectoderm, such as BALB/c 3T3 fibroblasts, capillaries and endocardial cells, myoblasts, vascular smooth muscle cells, mesothelial cells, astrocytes and glial cells, adrenal cortex, etc, it has a wide range of biological effects and potential clinical application value. The aFGF molecule contains a heparin binding region, which can increase the activity on some cells after binding of aFGF and heparin, and a certain amount of heparin can increase the stability of aFGF and prevent aFGF from being denatured by heat, extreme pH and proteolytic hydrolysis. Recombinant human acidic fibroblast growth factor (rh-aFGF) expressed in E. coli is a non-glycosylated single-chain protein containing 141 amino acids with a molecular weight of 16.0kDa.

Product features and technical indicators

Product Name

Recombinant human acidic fibroblast growth factor (Recombinant Human Fibroblast Growth Factor-acidic, rh-aFGF)

Catalog No.

NRPA15

Specifications

100 μg,1mg,10mg,100mg

Product form

Lyophilized powder, 10mM PBS (pH 6.0) before lyophilization

molecular weight

16.0kDa

isoelectric point

7.73
absorption coefficient UV absorption method, extinction coefficient E(0.1% at 280nm)= 1.091

Source

Escherichia coli

Purity

≥ 95%(SDS-PAGE detection)

endotoxin

<50 EU/mg

Save condition

-20 ℃ preservation
Conditions of transport Cold transport of ice packs or dry ice

Validity Period

3 years

activity

≥ 2.5 × 105 IU/mg protein (dose-dependent proliferation assay of mouse BALB/c 3T3 cells)