NRPA04S

Product Name:

Recombinant Staphylococcus aureus Protein A

Protein A is a Staphylococcus aureus cell wall protein that binds specifically to IgG and a few IgM and IgA of many mammals, primarily to the Fc region of immunoglobulin heavy chains. Protein A can be coupled with a variety of reporter molecules (fluorescent dyes, enzyme labels, biotin, colloidal gold, radioactive labels, etc.) without affecting its binding activity with antibodies, these coupling reactions can be used for ELISA assays, histochemistry, Blot-Western antibody detection. After protein A is combined with sepharose, an affinity filler for antibody purification can also be prepared, and antibodies of various mammalian subtypes or genetically engineered recombinant proteins containing antibody Fc fragments can be isolated and purified from ascites, serum, cell culture supernatant or cell extracts.

Product features and technical indicators

Product Name	Recombinant Staphylococcus aureus protein A(Recombinant Staphylococcal Protein A, r-SPA)
Catalog No.	NRPA04
Specifications	1 mg ,10 mg ,100 mg ,500 mg
Product form	Lyophilized powder; buffer system is 5mM PB(pH 7.4), 0.1mM EDTA solution before lyophilization
molecular weight	33.6kDa（SDS-PAGE 约30 kDa）
isoelectric point	5.10
activity	≥ 800 U/mg protein
Source	Escherichia coli
absorbance index	UV absorption method, extinction coefficient E(0.1% at 280 nm) = 0.221
Purity	≥95%
Save condition	-20 ℃ preservation
Conditions of transport	Cold transport of ice packs or dry ice
Validity Period	3 years

Key words:

Recombinant Staphylococcus aureus Protein A

Classification:

Ligand proteins

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Sales Phone：0571-82872376

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E-mail：marketing@nuptec.com