NRPA58S

Product Name:

Recombinant Core Streptavidin 4

Streptavidin (Streptavidin,SA) is a homotetrameric protein secreted by Streptomyces avidin (Streptomyces avidinii) during growth. Like avidin, one mole of streptavidin can bind four moles of biotin, with a high affinity for biotin. Since SA does not contain a sugar group and the isoelectric point is close to neutral, SA has a lower level of non-specific binding in detection applications than pro. Compared with natural streptavidin, the recombinant core streptavidin 4 removes the sequence unrelated to the activity, and only retains the core sequence related to the activity. The stability and solubility of the recombinant core streptavidin 4 are superior to natural streptavidin. The sequence was modified to increase the content of amino groups and improve the efficiency of coupling with ligands or magnetic beads by amino groups (pI:8.93). The product has been widely used in a variety of biotechnology fields, such as: coating microplates for immunodetection, preparing SA-coupled enzyme preparations, SA-coupled fluorescein, SA-coupled magnetic beads, etc., and then participating in enzyme-linked immunosorbent and enzyme catalytic amplification experiments, immunohistochemistry, biological molecule purification, biosensors, biological nanospheres, pre-targeted pharmaceutical research and biochip materials, etc.

Product features and technical indicators

Product Name	Recombinant Core Streptavidin 4(Recombinant Core Streptavidin 4, r-cSA4)
Catalog No.	NRPA58
Specifications	1 mg,10 mg,100 mg,500 mg
Product form	Freeze dried powder (5mM potassium phosphate buffer, trehalose, pH 7.4)
molecular weight	Tetramer 55.2kDa, Monomer 13.8kDa
activity	≥ 16 U/mg protein (determined by Green's modified method)
Purity	≥ 95%(SDS-PAGE detection)
concentration determination	UV absorption method, extinction coefficient E(0.1% at 280 nm) = 3.042
Source	Escherichia coli fermentation engineering strain
Save condition	-20 ℃ preservation
Conditions of transport	Cold transport of ice packs or dry ice
Validity Period	3 years

Note:

1. Streptavidin lyophilized powder is easily soluble in water, and the solubility can reach 10 mg/mL or higher;
2. It is recommended to use pure water for dissolution;
3. If there are undissolved substances, it is recommended to extend the redissolution time. The insoluble matter can also be removed by centrifugation or other means, and then used in subsequent experiments, which will not have a great impact on the total protein.
4. Considering the particularity of protein, freeze-dried powder is recommended to be used now, and repeated freezing and thawing should be avoided after dissolution. If it needs to be used many times, it is recommended to freeze and store (-20 ℃) after packaging as required. Avoid long-term storage at 4 ℃.

Key words:

Recombinant Core Streptavidin 4

Classification:

IVD proteins

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