NRPA44S

Product Name:

Sticky streptavidin

Streptavidin (Streptavidin,SA) is a homotetrameric protein secreted by Streptomyces avidin (Streptomyces avidinii) during growth. Like avidin, one mole of streptavidin can bind four moles of biotin, with a high affinity for biotin. Because SA does not contain sugar, SA has a lower non-specific binding level than pro-hormone in detection applications. It has been widely used in enzyme-linked immunosorbent assay, immunohistochemistry, time-resolved immunofluorescence (TRFIA), quantitative PCR, single-stranded DNA preparation, biological molecule purification, monoclonal antibody preparation and other biotechnology fields. However, the adsorption of streptavidin is poor, especially in practical applications, it is difficult to adsorb to NC membrane or embed. The viscous recombinant streptavidin (S-rSA) is mainly to solve this problem, which not only has the core sequence related to activity, but also is superior to natural SA in adsorption, stability and solubility, especially in NC membrane.

Product features and technical indicators

Product Name	粘性链霉亲和素（Recombinant Sticky Core Streptavidin, r-ScSA）
Catalog No.	NRPA44
Specifications	1 mg,10 mg,100 mg,500 mg,1g
Product form	Lyophilized powder, 5mM PB (4mM Na2HPO4, 1mM NaH2PO4, pH 7.4) before lyophilization
molecular weight	Subunit monomer 23.5kDa, tetramer 94kDa
isoelectric point	6.04
activity	≥ 14 U/mg protein (determined by Green's modified method)
Purity	≥ 95%(SDS-PAGE detection)
concentration determination	UV absorption method, extinction coefficient A280 = 3.136
endotoxin	≤ 5EU/mg
Source	Escherichia coli
Save condition	Storage at ≤-20 ℃
Conditions of transport	Cold transport of ice packs or dry ice
Validity Period	3 years

Key words:

Sticky streptavidin

Classification:

IVD proteins

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Sales Phone：0571-82872376

Business Phone：0571-82872986

E-mail：marketing@nuptec.com