NRPA34S

Product Name:

Recombinant Streptavidin 3

Streptavidin (Streptavidin,SA) and biotin have high affinity, affinity constant Kd can generally reach 10-15M, active SA usually exists in the form of tetramer. Because SA does not contain sugar, SA has a lower non-specific binding level than pro-hormone in detection applications. It has been widely used in enzyme-linked immunosorbent assay, immunohistochemistry, time-resolved immunofluorescence (TRFIA), quantitative PCR, single-stranded DNA preparation, biological molecule purification, monoclonal antibody preparation and other biotechnology fields. However, due to the ultra-high affinity of SA and biotin, it is generally difficult to separate the combined SA and biotin by mild methods. The conditions under which SA can be separated will generally make SA inactive. Similarly, biotin-labeled antibodies and antigens will also lose their activity. Therefore, it is generally believed that the binding of SA and biotin is irreversible. Recombinant streptavidin 3(r-SA3) is modified at the molecular level on the basis of natural streptavidin to greatly weaken the affinity with biotin (Kd ≈ 10-8M), while maintaining the tetramer structure, which can realize reversible binding and dissociation with biotin, and can be used for mild separation, purification and recycling of biotinylated proteins, antibodies, nucleic acids and other substances. r-SA3 containing cysteine in the molecule, directional coupling can be achieved under certain conditions.

Product features and technical indicators

Product Name	Recombinant streptavidin 3(Recombinant Streptavidin 3, r-SA3)
Catalog No.	NRPA34
Specifications	1 mg,10 mg,100 mg,500 mg,1g
NRPA34	Lyophilized powder, 5mM PB(pH 7.4) solution before lyophilization
molecular weight	16.7 kDa
isoelectric point	6.06
Purity	≥ 95%(SDS-PAGE detection)
concentration determination	UV absorption method, extinction coefficient E(0.1% at 280 nm) = 2.51
Source	Escherichia coli
Save condition	-20 ℃ preservation
Conditions of transport	Cold transport of ice packs or dry ice
Validity Period	3 years

Key words:

Recombinant Streptavidin 3

Classification:

IVD proteins

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