NRPA32S

Product Name:

SUMO protease (GST tag)

SUMO protease 1(Small Ubiquitin-like MOdifiers Protease 1), also known as Ulp1(Ubl-specific protease 1), is a highly active cysteine protease found in Saccharomyces cerevisiae, which can specifically recognize the tertiary structure of the ubiquitin-like protein (SUMO protein), rather than the amino acid sequence, and specific cleavage occurs. SUMO protein is often used as a fusion tag, which can effectively improve the solubility, stability and expression of the target protein in the prokaryotic expression system (or eukaryotic expression system). SUMO tag fusion protein can be cleaved by SUMO protease 1 after expression and purification. SUMO protease 1 cleaves after two consecutive glycine sequences (-Gly-Gly) at the C- terminal of SUMO tag. Reasonable design can make the N-terminal of the cleaved target protein free of any excess amino acids. SUMO protease 1(GST tag) The GST tag was added to the N-terminal of the amino acid sequence of SUMO protease 1, which was expressed by E. coli and purified by a variety of chromatographic methods to obtain high-purity SUMO protease 1. The optimal reaction temperature of the enzyme is 30 degrees C, but in certain reaction conditions (4-37 degrees, pH5-9 degrees, etc.) can maintain efficient cleavage activity, fusion of GST tag, easy to target protein cleavage after the use of GSTAffinity chromatograph to remove the enzyme.

Product features and technical indicators

Product Name	SUMO蛋白酶（GST标签）（SUMO Protease , GST-tag; SUMO-P(GST)）
Catalog No.	NRPA32
Specifications	1mg，10mg，100mg，500mg
Product form	Lyophilized powder, 5 mM PB(pH 7.4), 2mM DTT before lyophilization
molecular weight	52.5 kDa
isoelectric point	5.79
specific activity	≥50. 000u/mg
absorption coefficient	UV absorption method, extinction coefficient E(0.1% at 280 nm) = 1.394
Source	Escherichia coli
Purity	≥ 85%(SDS-PAGE detection)
Save condition	-20 ℃ preservation
Conditions of transport	Cold transport of ice packs or dry ice
Validity Period	3 years
Activity Definition	In a buffer system at pH 8.0 (50mM Tris-HCl, or 20mM PB), the amount of enzyme required to cleave> 85% of 2 μg of substrate for 1h at 30°C is defined as 1 activity unit.

Key words:

SUMO protease (GST tag)

Classification:

Enzyme serials

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E-mail：marketing@nuptec.com