NRPA19S

Product Name:

Recombinant Bovine Enterokinase (Histidine Tag)

Enterokinase (EC 3.4.21.9) is a serine proteolytic enzyme, which can efficiently and specifically identify the sequence of Asp-Asp-Asp-Asp-Lys in protein (DDDDK), hydrolyze peptide bonds at the C- terminal of lysine (Lys,K), produce cleavage, and hydrolyze trypsinogen into trypsin in vivo. Enterokinase is widely used in the development of genetic engineering products due to its high specificity and efficient enzyme cleavage characteristics. Natural enterokinase is composed of a 115kDa heavy chain and a 35kDa light chain, the heavy chain plays the role of anchoring the cell membrane, and the light chain has the complete catalytic activity of the whole enzyme. This product is a fusion protein of the core region of the light chain activity of bovine enterokinase and histidine tag, expressed by Escherichia coli secretion technology, and has higher specific activity than natural enterokinase. At the same time with a histidine tag, after the enzyme can be used nickel column (NRPB07) to remove the rbEK-His in the sample, especially suitable for genetic engineering fusion protein enzyme digestion.

Product features and technical indicators

Product Name	Recombinant bovine enterokinase (histidine tag)(Recombinant Bovine Enterokinase, His-tag; r-bEK-His)
Catalog No.	NRPA19
Specifications	200 U (liquid),1000 U (liquid),100 μg,1 mg
Product form	Liquid is 50mM Tris-HCl(pH 8.0), 50% glycerol; Lyophilized powder is 50mM Tris-HCl(pH 8.0) solution before lyophilization
molecular weight	28.1 kDa (approx. 30 kDa for SDS-PAGE detection)
isoelectric point	5.85
specific activity	≥ 40000 U/mg, I .e. 1mg rbEK-His cleaves at least 2000mg of fusion protein.
absorption coefficient	UV absorption method, extinction coefficient E(0.1% at 280 nm) = 1.92
Source	Escherichia coli
Conditions of transport	Cold transport of ice packs or dry ice
Save condition	-20 ℃ preservation
Validity Period	3 years

Note:

(1) Some reagents will affect the activity of the enzyme, such as> 2M Urea,>250mM NaCl,>20mM β-ME,>0.1% SDS,>50mM Imidazole. If the sample contains these components, it needs to be dialyzed to 50mM Tris-HCl (pH 8.0).
(2) Phosphate can inhibit the activity of enterokinase, and phosphate buffer should be avoided as an enzyme digestion system.

Key words:

Recombinant Bovine Enterokinase (Histidine Tag)

Classification:

Enzyme serials

Download Product Description

Online Preview

Tel: 0571-82872376

E-mail: marketing@nuptec.com

Alibaba official website mall

Get Quote

COA Download

Apply for a trial outfit

COOKIES

Our website uses cookies and similar technologies to personalize the advertising shown to you and to help you get the best experience on our website. For more information, see our Privacy & Cookie Policy

COOKIES

required

These cookies are necessary for basic functions such as payment. Standard cookies cannot be turned off and do not store any of your information.

analyze

These cookies collect information, such as how many people are using our site or which pages are popular, to help us improve the customer experience. Turning these cookies off will mean we can't collect information to improve your experience.

Feature

These cookies enable the website to provide enhanced functionality and personalization. They may be set by us or by third-party providers whose services we have added to our pages. If you do not allow these cookies, some or all of these services may not function properly.

advertise

These cookies help us understand what you are interested in so that we can show you relevant advertising on other websites. Turning these cookies off will mean we are unable to show you any personalized advertising.

NUPTEC Inc. Ltd.

Sales Phone:0571-82872376
Business Phone:0571-82872986
E-mail:marketing@nuptec.com
Headquarters Address: Building 25, Qianwan Zhiqi, Jiangnan Science and Technology City, Hangzhou City, Zhejiang Province
Production Base of Li: No.63, No.5 Road, Dangyuan, Li Town, Xiao District, Hangzhou City, Zhejiang Province
Linjiang Production Base: 698 Jing Lu, Linjiang Street, Qiantang District, Hangzhou City, Zhejiang Province

Online Message →

Sales Phone：0571-82872376

Business Phone：0571-82872986

E-mail：marketing@nuptec.com