NRPB131L

Product Name:

NUPhadex G-25 SF

NUPhadex G-25 series molecular sieve filler is epichlorohydrin cross-linked dextran microspheres. The non-specific adsorption of dextran to biomolecules is very low, hydrophilic, porous crosslinked dextran microspheres are mainly used for rapid liquid exchange of biomolecules, desalination and other group separation processes, with a small degree of sample dilution, desalination rate and high recovery rate. The exclusion limit of this product for globulin is about 5000 Da. The retention time of biomolecules with relatively large molecular weight in the filler is short, and the retention time of salts and other small molecules in the buffer is long. Molecules of different sizes are separated due to the different residence time in the filler. This product is suitable for various biomolecule samples such as protein, polysaccharide, nucleic acid, etc. The fractionation process may also be attempted to isolate and purify biomolecules such as polypeptides, oligonucleotides, antibiotics, and natural products with small molecular weight differences. The particle size of this product has three specifications, namely NUPhadex G-25 SF(Superfine, ultrafine grain), NUPhadex G-25 M(Medium, medium grain) and NUPhadex G-25 C(Coarse, coarse grain). This product has outstanding pressure and flow rate performance, and SF grade can replace other S grade (Fine, fine) products on the market. Unlike most products on the market that only provide dry powder, this product also provides swollen microspheres.

Product features and technical indicators

Product Name	NUPhadex G-25 SF	NUPhadex G-25 M	NUPhadex G-25 C
Catalog No.	NRPB131L、 NRPB131S (pre-mounted column)	NRPB132L、 NRPB132S (pre-mounted column)	NRPB133L、 NRPB133S (pre-mounted column)
Appearance	Milky White a gel microsphere dispersion, a powder is also provided.
Dry particle size range^a, μm	20~60	50~150	100~300
Swelling particle size range^a, μm	30~100	80~250	170~500
Pre-mounted column products	MV pre-installed column Y series 5 ml, φ/h: 16 × 25 mm	Gravity preloaded column Z series (8 mL) Centrifugal preloaded column L series (0.6 mL)	Medium pressure pre-installed column S series 53 ml, φ/h: 26 × 100 mm
Swelling Gel/Dry Powder (v/wt)	4~6 mL/g
Globulin separation range, Da	100~5000
Maximum flow rate^b，cm/h	≥300	≥ 600	≥800
pH stability	2~13
chemical stability	It is stable in various buffer systems, such as 6 mol/L guanidine hydrochloride, 1 mol/L acetic acid, 8 mol/L urea, 30% isopropanol, 0.5 mol/L NaOH.
Sterilization	pH = 7, autoclaving at 121 ℃ for 30 min
Storage and transportation	2~30 ℃, containing 20% ethanol or dry powder
Shelf life	Dispersion 3 years, dry powder 5 years

Key words:

NUPhadex G-25 SF

Classification:

Gel filtration

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NUPTEC Inc. Ltd.

Sales Phone:0571-82872376
Business Phone:0571-82872986
E-mail:marketing@nuptec.com
Headquarters Address: Building 25, Qianwan Zhiqi, Jiangnan Science and Technology City, Hangzhou City, Zhejiang Province
Production Base of Li: No.63, No.5 Road, Dangyuan, Li Town, Xiao District, Hangzhou City, Zhejiang Province
Linjiang Production Base: 698 Jing Lu, Linjiang Street, Qiantang District, Hangzhou City, Zhejiang Province

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Sales Phone：0571-82872376

Business Phone：0571-82872986

E-mail：marketing@nuptec.com