NUPTEC Inc. Ltd.

Product Details

Professional Manufaturer of Resins for Protein Purification

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Recombinant Core Streptavidin 2
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  • Recombinant Core Streptavidin 2

Catalog No:

NRPA18S / NRPA18 V1S

Product Name:

Recombinant Core Streptavidin 2


Streptavidin (Streptavidin,SA) is a homotetrameric protein secreted by Streptomyces avidin (Streptomyces avidinii) during growth. Like avidin, one mole of streptavidin can bind four moles of biotin, with a high affinity for biotin. Since streptavidin does not contain sugar groups and the isoelectric point is close to neutral, streptavidin has a lower level of non-specific binding in detection applications than pro-biotin. Compared with the natural streptavidin, the recombinant core streptavidin 2 removes the sequence unrelated to the activity, only retains the core sequence related to the activity, and is superior to the natural streptavidin in stability and solubility. (The recombinant streptavidin 2 pI:6.09) The product has been widely used in a variety of biotechnology fields, such as: coating microplates for immunodetection, preparing SA-coupled enzyme preparations, SA-coupled fluorescein, SA-coupled magnetic beads, etc., and then participating in enzyme-linked immunosorbent and enzyme catalytic amplification experiments, immunohistochemistry, biological molecule purification, biosensors, biological nanospheres, pre-targeted pharmaceutical research and biochip materials, etc.

Product features and technical indicators

Product Name

Recombinant Core Streptavidin 2(Recombinant Core Streptavidin 2, r-cSA2)

Catalog No.

NRPA18 / NRPA18 V1

Specifications

1 mg,10 mg,100 mg,500 mg

NRPA18

Freeze dried powder (5mM PB,pH 7.4)

NRPA18V1

Freeze dried powder (5mM HEPES buffer, trehalose, TCEP,pH 8.0)

molecular weight

Tetramer 53.2kDa, Monomer 13.3kDa

activity

≥ 16 U/mg protein (determined by Green's modified method)

Purity

≥ 95%(SDS-PAGE detection)

concentration determination

UV absorption method, extinction coefficient E(0.1% at 280 nm) = 3.16

Source

Escherichia coli fermentation engineering strain

Save condition

Storage at ≤ -20 ℃

Conditions of transport

Cold transport of ice packs or dry ice

Validity Period

3 years

Note:

1. Recombinant streptavidin 2 lyophilized powder is easily soluble in water, and the solubility can reach 10 mg/mL or higher;
2. It is recommended to use pure water for dissolution;
3. If there are undissolved substances, it is recommended to extend the redissolution time. The insoluble matter can also be removed by centrifugation or other means, and then used in subsequent experiments, which will not have a great impact on the total protein.
4. Considering the particularity of protein, freeze-dried powder is recommended to be used now, and repeated freezing and thawing should be avoided after dissolution. If it needs to be used many times, it is recommended to freeze and store (≤-20 ℃) after subpackaging as required; Avoid long-term storage at 4 ℃.